PDF(Pubmed)
Abstract：
Salicylic acid (SA) is an essential phytoregulator that is widely used to promote the synthesis of high-value nutraceuticals in plants. However, its application in daylily, an ornamental plant highly valued in traditional Chinese medicine, has not been reported. Herein, we investigated the exogenous SA-induced physiological, transcriptional and biochemical changes in long yellow daylily (LYD). We found that 2 mg/L foliar SA treatment significantly improved LYD plant growth and yield. Transcriptome sequencing and differentially expressed genes (DEGs) analysis revealed that the phenylpropanoid biosynthesis, isoquinoline alkaloid biosynthesis, sulfur metabolism, plant hormone signal transduction and tyrosine metabolism were significantly induced in SA-treated leaves. Many transcription factors and antioxidant system-related DEGs were induced under the SA treatment. Biochemical analyses showed that the leaf contents of soluble sugar, soluble protein (Cpr), ascorbic acid (AsA) and colchicine were significantly increased by 15.15% (from 30.16 ± 1.301 to 34.73 ± 0.861 mg/g), 19.54% (from 60.3 ± 2.227 to 72.08 ± 1.617 mg/g), 30.45% (from 190.1 ± 4.56 to 247.98 ± 11.652 μg/g) and 73.05% (from 3.08 ± 0.157 to 5.33 ± 0.462 μg/g), respectively, under the SA treatment. Furthermore, we identified 15 potential candidate genes for enhancing the growth, production and phytochemical content of LYD. Our results provide support for the bioaccumulation of colchicine in yellow daylily and valuable resources for biotechnological-assisted production of this important nutraceutical in Hemerocallis spp.
摘要：
水杨酸（SA）是一种重要的植物调节剂，广泛用于促进植物中高价值营养品的合成。然而,它在黄花菜中的应用，一种在中药中很有价值的观赏植物，尚未报告。在这里，我们研究了外源性SA诱导的生理，长黄花菜（LYD）的转录和生化变化。我们发现2mg/L叶面SA处理显着提高了LYD植物的生长和产量。转录组测序和差异表达基因（DEGs）分析显示，苯丙烷生物合成，异喹啉生物碱生物合成，硫代谢，在SA处理的叶片中显着诱导了植物激素信号转导和酪氨酸代谢。在SA处理下诱导了许多转录因子和抗氧化系统相关的DEGs。生化分析表明，叶片中可溶性糖的含量，可溶性蛋白（Cpr），抗坏血酸（AsA）和秋水仙碱显着增加了15.15％（从30.16±1.301到34.73±0.861mg/g），19.54%（从60.3±2.227到72.08±1.617mg/g），30.45％（从190.1±4.56到247.98±11.652μg/g）和73.05％（从3.08±0.157到5.33±0.462μg/g），分别,在SA治疗下。此外，我们确定了15个潜在的候选基因来促进生长，LYD的产量和植物化学含量。我们的结果为秋水仙碱在黄色黄花菜中的生物积累提供了支持，也为在Hemerocallisspp中生物技术辅助生产这种重要的营养食品提供了宝贵的资源。
