背景:软骨肉瘤是通过分析组织活检的放射学图像和组织学并评估基质钙化等特征来诊断的罕见恶性骨肿瘤,皮质破坏,小梁渗透,和肿瘤细胞截留。
方法:我们回顾性分析了3例患者的16个软骨肿瘤组织样本(51,54-,和70岁)诊断为股骨去分化软骨肉瘤,骨盆中度分化的软骨肉瘤,肩胛骨上有一个主要的中分化软骨肉瘤,分别。我们将基于血红素的X射线染色与高分辨率三维(3D)显微X射线计算机断层扫描(micro-CT)相结合,用于无损3D肿瘤评估和肿瘤边缘评估。
结果:我们在3D显微CT图像上检测到小梁截留,并跟踪整个体积的骨破坏。除了染色细胞核,基于血红素的染色也改善了肿瘤基质的可视化,允许区分肿瘤和骨髓腔。基于血红素的染色不干扰进一步的常规组织学。使用显微CT和组织病理学测量的相对肿瘤面积之间存在5.97±7.17%的差异(p=0.806)(皮尔逊相关系数r=0.92,p=0.009)。与未染色的对应物(1.92±0.11,p=0.002)相比,染色样品中肿瘤基质中的信号强度(4.85±2.94)显著更高。
结论:使用无损3DMicro-CT,放射学和组织病理学特征的同时可视化是可行的。
结论:3Dmicro-CT数据支持人类骨肿瘤标本的现代放射学和组织病理学研究。它有可能成为临床术前诊断的组成部分。
结论:•基质钙化是骨肿瘤的相关诊断特征。•Micro-CT检测X线染色软骨肉瘤的所有临床诊断相关特征。•Micro-CT有可能成为临床诊断的整合部分。
BACKGROUND: Chondrosarcomas are rare malignant bone tumors diagnosed by analyzing radiological images and histology of tissue biopsies and evaluating features such as matrix calcification, cortical destruction, trabecular penetration, and tumor cell entrapment.
METHODS: We retrospectively analyzed 16 cartilaginous tumor tissue samples from three patients (51-, 54-, and 70-year-old) diagnosed with a dedifferentiated chondrosarcoma at the femur, a moderately differentiated chondrosarcoma in the pelvis, and a predominantly moderately differentiated chondrosarcoma at the scapula, respectively. We combined a
hematein-based x-ray staining with high-resolution three-dimensional (3D) microscopic x-ray computed tomography (micro-CT) for nondestructive 3D tumor assessment and tumor margin evaluation.
RESULTS: We detected trabecular entrapment on 3D micro-CT images and followed bone destruction throughout the volume. In addition to staining cell nuclei,
hematein-based staining also improved the visualization of the tumor matrix, allowing for the distinction between the tumor and the bone marrow cavity. The
hematein-based staining did not interfere with further conventional histology. There was a 5.97 ± 7.17% difference between the relative tumor area measured using micro-CT and histopathology (p = 0.806) (Pearson correlation coefficient r = 0.92, p = 0.009). Signal intensity in the tumor matrix (4.85 ± 2.94) was significantly higher in the stained samples compared to the unstained counterparts (1.92 ± 0.11, p = 0.002).
CONCLUSIONS: Using nondestructive 3D micro-CT, the simultaneous visualization of radiological and histopathological features is feasible.
CONCLUSIONS: 3D micro-CT data supports modern radiological and histopathological investigations of human bone tumor specimens. It has the potential for being an integrative part of clinical preoperative diagnostics.
CONCLUSIONS: • Matrix calcifications are a relevant diagnostic feature of bone tumors. • Micro-CT detects all clinically diagnostic relevant features of x-ray-stained chondrosarcoma. • Micro-CT has the potential to be an integrative part of clinical diagnostics.