METHODS: We retrospectively analyzed 16 cartilaginous tumor tissue samples from three patients (51-, 54-, and 70-year-old) diagnosed with a dedifferentiated chondrosarcoma at the femur, a moderately differentiated chondrosarcoma in the pelvis, and a predominantly moderately differentiated chondrosarcoma at the scapula, respectively. We combined a hematein-based x-ray staining with high-resolution three-dimensional (3D) microscopic x-ray computed tomography (micro-CT) for nondestructive 3D tumor assessment and tumor margin evaluation.
RESULTS: We detected trabecular entrapment on 3D micro-CT images and followed bone destruction throughout the volume. In addition to staining cell nuclei, hematein-based staining also improved the visualization of the tumor matrix, allowing for the distinction between the tumor and the bone marrow cavity. The hematein-based staining did not interfere with further conventional histology. There was a 5.97 ± 7.17% difference between the relative tumor area measured using micro-CT and histopathology (p = 0.806) (Pearson correlation coefficient r = 0.92, p = 0.009). Signal intensity in the tumor matrix (4.85 ± 2.94) was significantly higher in the stained samples compared to the unstained counterparts (1.92 ± 0.11, p = 0.002).
CONCLUSIONS: Using nondestructive 3D micro-CT, the simultaneous visualization of radiological and histopathological features is feasible.
CONCLUSIONS: 3D micro-CT data supports modern radiological and histopathological investigations of human bone tumor specimens. It has the potential for being an integrative part of clinical preoperative diagnostics.
CONCLUSIONS: • Matrix calcifications are a relevant diagnostic feature of bone tumors. • Micro-CT detects all clinically diagnostic relevant features of x-ray-stained chondrosarcoma. • Micro-CT has the potential to be an integrative part of clinical diagnostics.
方法:我们回顾性分析了3例患者的16个软骨肿瘤组织样本(51,54-,和70岁)诊断为股骨去分化软骨肉瘤,骨盆中度分化的软骨肉瘤,肩胛骨上有一个主要的中分化软骨肉瘤,分别。我们将基于血红素的X射线染色与高分辨率三维(3D)显微X射线计算机断层扫描(micro-CT)相结合,用于无损3D肿瘤评估和肿瘤边缘评估。
结果:我们在3D显微CT图像上检测到小梁截留,并跟踪整个体积的骨破坏。除了染色细胞核,基于血红素的染色也改善了肿瘤基质的可视化,允许区分肿瘤和骨髓腔。基于血红素的染色不干扰进一步的常规组织学。使用显微CT和组织病理学测量的相对肿瘤面积之间存在5.97±7.17%的差异(p=0.806)(皮尔逊相关系数r=0.92,p=0.009)。与未染色的对应物(1.92±0.11,p=0.002)相比,染色样品中肿瘤基质中的信号强度(4.85±2.94)显著更高。
结论:使用无损3DMicro-CT,放射学和组织病理学特征的同时可视化是可行的。
结论:3Dmicro-CT数据支持人类骨肿瘤标本的现代放射学和组织病理学研究。它有可能成为临床术前诊断的组成部分。
结论:•基质钙化是骨肿瘤的相关诊断特征。•Micro-CT检测X线染色软骨肉瘤的所有临床诊断相关特征。•Micro-CT有可能成为临床诊断的整合部分。