背景:Harmoniaaxyridis是各种韧皮部吸吮害虫和鳞翅目幼虫的有效天敌昆虫,比如蚜虫,疮,和根瘤菌,而由于人工饮食不成熟,其工业生产受到限制。昆虫肠道菌群影响宿主发育和繁殖。本研究的目的是了解轴孢菌的肠道菌群组成,筛选人工饮食中有效的益生菌。考虑到饮食的成分和组成对肠道微生物组的结构和组成的作用,设置了四种饮食:(1)蚜虫;(2)基本饮食;(3)基本饮食葡萄糖;(4)基本饮食海藻糖。在饲喂不同饮食后,检测到了H.axyridis的肠道菌群。
结果:结果表明,人工饮食组和蚜虫组之间的肠道菌群相距甚远,而基础组和葡萄糖组明显聚集。此外,葡萄糖组和海藻糖组有一个独特的门,隐球菌和念珠菌,分别。在蚜虫饮食中发现了最高丰度的变形杆菌。在基本饮食中发现了最高丰度的Firmicutes。然而,葡萄糖或海藻糖的加入减轻了这种变化。此外,肠杆菌的相对丰度,克雷伯菌属,肠杆菌科_未分类,蚜虫组中未分类的肠杆菌和沙雷氏菌高于其他组。此外,各组肠道基因功能也有明显差异。
结论:这些结果提供了人工饮食与肠道微生物之间的紧密联系,为人工日粮中协同益生菌的筛选提供了理论依据。
BACKGROUND: Harmonia axyridis is an effective natural enemy insect to a variety of phloem-sucking pests and Lepidopteran larvae, such as aphids, scabies, and phylloxera, while its industrial production is limited due to unmature artificial diet. Insect intestinal microbiota affect host development and reproduction. The aim of this study is to understand intestinal microbiota composition of H. axyridis and screen effective probiotics on artificial diet. Considering the role of the components and composition of the diet on the structure and composition of the intestinal microbiome, four kinds of diets were set up: (1) aphid; (2) basic diet; (3) basic diet + glucose; (4) basic diet + trehalose. The gut microbiota of H. axyridis was detected after feeding on different diets.
RESULTS: Results showed that the gut microbiota between artificial diet group and aphid groups were far apart, while the basic and glucose groups were clearly clustered. Besides, the glucose group and trehalose group had one unique phylum, Cryptophyta and Candidatus Saccharibacteria, respectively. The highest abundance of Proteobacteria was found in the aphid diet. The highest abundance of Firmicutes was found in the basic diet. However, the addition of glucose or trehalose alleviated the change. In addition, the relative abundance of Enterobacter, Klebsiella, Enterobacteriaceae_unclassified, Enterobacteriales_unclassified and Serratia in the aphid group was higher than other groups. Moreover, the function of gut genes in each group also showed clear differences.
CONCLUSIONS: These results have offered a strong link between artificial diets and gut microbes, and also have provided a theoretical basis for the screening of synergistic probiotics in artificial diet.