豌豆(PisumsativumL,2n=14)是一种具有较高经济价值的风行温带豆科植物。热休克因子(HSF)是热应激反应调控机制的核心要素。豌豆中的HSF(P.sativum)尚未被表征,它们在不同非生物胁迫中的作用尚不清楚。为了解决这个知识差距,本研究旨在表征豌豆中的HSF基因家族。我们在沙棘中确定了38个PsHsf成员,它们分布在七个染色体上,根据系统发育分析,我们将它们分为三个有代表性的类别,即A,B,保守基序和基因结构分析证实了同一类成员之间的高度相似性。此外,确定的与非生物反应相关的顺式作用调控元件(CARE),发展,增长,和激素信号传导为PsHsfs的调节机制提供了重要的见解。我们的研究揭示了PsHsf基因家族中基因复制的实例,这表明这种机制可能推动了PsHsf基因家族的扩展。此外,PsHsfs的表达分析在热应激(HS)下表现出上调,盐胁迫(SS),和干旱胁迫(DS)在胁迫条件下显示出显著的作用。PsHsfs蛋白相互作用网络表明它们参与了应激反应机制。进一步检查了PsHsfA2a的剪接变体(PsHsfA2aI,PsHsfA2aII,和PsHsfA2aIII),PsHsfA3,PsHsfA6b,PsHsfA9,PsHsfB1a,和PsHsfB2a。总的来说,这些发现为PsHsf基因家族的进化关系及其在非生物胁迫反应中的作用提供了有价值的见解。
Field pea (Pisum sativum L, 2n = 14) is a popular temperate legume with high economic value. Heat shock factors (HSFs) are the core element in the regulatory mechanism of heat stress responses. HSFs in pea (P. sativum) have not been characterized and their role remains unclear in different abiotic stresses. To address this knowledge gap, the current study aimed to characterize the
HSF gene family in pea. We identified 38 PsHsf members in P. sativum, which are distributed on the seven chromosomes, and based on phylogenetic analysis, we classified them into three representative classes i.e. A, B, and C. Conserved motif and gene structure analysis confirmed a high degree of similarity among the members of the same class. Additionally, identified cis-acting regulatory elements (CAREs) related to abiotic responses, development, growth, and hormone signaling provides crucial insights into the regulatory mechanisms of PsHsfs. Our research revealed instances of gene duplication in PsHsf gene family, suggesting that this mechanism could be driving the expansion of the PsHsf gene family. Moreover, Expression analysis of PsHsfs exhibited upregulation under heat stress (HS), salt stress (SS), and drought stress (DS) showing their phenomenal role in stress conditions. PsHsfs protein interaction network suggested their involvement in stress-responsive mechanisms. Further transactivation potential was checked for spliced variant of PsHsfA2a (PsHsfA2aI, PsHsfA2aII, and PsHsfA2aIII), PsHsfA3, PsHsfA6b, PsHsfA9, PsHsfB1a, and PsHsfB2a. Overall, these findings provide valuable insight into the evolutionary relationship of PsHsf gene family and their role in abiotic stress responses.