Abstract：
Trilinolein (TL) is an active substance contained in traditional Chinese herbs; modern studies have shown that trilinolein has anti-inflammatory and antioxidant effects on the body. This study delves into the photoprotective effect of trilinolein on UVB-irradiated Human Skin Fibroblast (HSF) cells and the underlying mechanisms. Our findings reveal that trilinolein had a photoprotective effect on HSF cells: trilinolein enhanced cellular autophagy, restored UVB-inhibited cell proliferative viability, and curbing UVB-induced reactive oxygen species (ROS) and apoptosis. Intriguingly, after inhibition of TL-induced autophagy via wortmannin, diminished trilinolein\'s photoprotective effects. Meanwhile, trilinolein was shown to modulate the AMPK-mTOR signaling pathway, thus enhance cellular autophagy in HSF cells, and this tendency was suppressed after the administration of compound C (AMPK inhibitor). In a mouse model of skin photodamage, trilinolein significantly mitigated photodamage extent through morphological and histopathological analyses. This study illuminates trilinolein could inhibit the photodamaging effects of UVB irradiation by regulating cellular autophagy through the AMPK-mTOR signaling pathway, suggesting its promising application in combating UV-induced skin disorders.
摘要：
Trilinolein（TL）是传统中草药中含有的活性物质；现代研究表明，trilinolein对人体具有抗炎和抗氧化作用。本研究探讨了三烯油苷对UVB照射的人皮肤成纤维细胞（HSF）的光保护作用及其机制。我们的发现揭示了三维醇苷对HSF细胞具有光保护作用：三维醇苷增强了细胞自噬，恢复UVB抑制的细胞增殖活力，并抑制UVB诱导的活性氧（ROS）和细胞凋亡。有趣的是，通过wortmannin抑制TL诱导的自噬后，三烯酚苷的光保护作用减弱。同时,显示trilinoolein调节AMPK-mTOR信号通路，从而增强HSF细胞中的细胞自噬，在给予化合物C(AMPK抑制剂)后,这种倾向得到抑制。在皮肤光损伤的小鼠模型中，通过形态学和组织病理学分析，三烯油苷可显着减轻光损伤程度。本研究通过AMPK-mTOR信号通路调节细胞自噬,从而抑制UVB照射的光损伤效应,表明其在对抗紫外线引起的皮肤病中的有希望的应用。
