动物废物是一种潜在的污染危害,因为它可能含有污染物,如抗菌残留物,霉菌毒素,和杀虫剂,成为公众的风险,动物,和环境健康。为了评估这种风险,15只实验肉鸡口服接受污染物以评估排泄水平。先前开发了一种分析方法,该方法使用高效液相色谱和串联质谱仪(HPLC-MS/MS)检测家禽粪便中的18种物质。污染物包括四环素,4-表四环素,土霉素,4-表-土霉素,金霉素,4-表-金霉素,泰乐菌素,红霉素,恩诺沙星,环丙沙星,氟美喹,氟苯尼考,磺胺氯吡啶,磺胺嘧啶,2,4-二氯苯氧基乙酸,玉米赤霉烯酮,α-和β-玉米赤霉烯醇,用EDTA-McIlvain和乙腈提取。该方法显示p值<0.05,RSD<25%,并且所有分析物的校准曲线线性中的R2>0.95。定量的极限,选择性,确认的决定限制,基体效应,精度,和恢复参数根据欧盟文件2021/808/EC进行了验证,技术报告CEN/TR16059,SANTE/11813/2017和根据兽医国际协调会议:VICHGL2和GL49。该方法证实了在给药后12-36小时检测到大多数分析物,并且同时检测和定量混合污染物。因此,家禽粪便是屠宰前在动物生产中传播污染物的潜在基质,对其进行控制将最大程度地减少对环境的影响并减轻抗菌素耐药性。
Animal waste is a potential pollution hazard as it can harbour contaminants, such as antimicrobial residues, mycotoxins, and pesticides, becoming a risk to the public, animal, and environmental health. To assess this risk, 15 experimental broiler chickens orally received contaminants to evaluate excretion levels. An analytical method was previously developed to detect 18 substances in poultry droppings using high-performance liquid chromatography coupled to a tandem mass spectrometer (HPLC-MS/MS). Contaminants including tetracycline, 4-epi-tetracycline, oxytetracycline, 4-epi-oxytetracycline, chlortetracycline, 4-epi-chlortetracycline, tylosin, erythromycin, enrofloxacin, ciprofloxacin, flumequine, florfenicol, sulfachloropyridazine, sulfadiazine, 2,4-dichlorophenoxyacetic acid, zearalenone, alpha- and beta-zearalenol, were extracted with EDTA-McIlvain and acetonitrile. This method showed a p-value < 0.05, RSD < 25%, and R2 > 0.95 in the calibration curves linearity for all analytes. The limit of quantification, selectivity, decision limit for confirmation, matrix effect, precision, and recovery parameters were validated according to European Union document 2021/808/EC, technical report CEN/TR 16059, SANTE/11813/2017 and according to the Veterinary International Conference on Harmonization: VICH GL2 and GL49. This method confirmed the detection of most analytes 12-36 h post-administration and simultaneously detected and quantified mixed contaminants. Thereby, poultry droppings are a potential matrix for spreading contaminants in animal production before slaughter and their control will minimize environmental impacts and mitigate antimicrobial resistance.