目的:最近的研究表明,HOTTIP和MEG3与各种类型肿瘤的发生和进展有关,包括鼻咽癌(NPC)。本研究旨在阐明HOTTIP和MEG3多态性对鼻咽癌易感性和临床病理特征的影响。
方法:本研究采用下一代测序和多重PCR分别在200例鼻咽癌和200例健康个体中评估HOTTIPrs1859168和MEG3rs7158663的多态性。通过qRT-PCR评估评估HOTTIP和MEG3表达。此外,在健康个体和NPC个体之间比较了rs1859168和rs7158663的基因型和等位基因频率,以阐明它们对NPC易感性的影响以及与临床病理特征的关系。
结果:与健康队列相比,HOTTIPrs1859168CC基因型和C等位基因的存在与NPC发病率增加显著相关(p<0.05)。此外,MEG3rs7158663AA基因型和A等位基因也表明NPC的风险增加(p<0.05)。年龄的亚组分析,EBV感染,性别,国籍,吸烟,饮酒状态显示rs1859168和rs7158663基因型与这些潜在的混杂因素之间没有显着关联。此外,观察到rs1859168CC和rs7158663AA基因型与局部肿瘤浸润和淋巴结转移有关。此外,HOTTIP标出了标高,而NPC样本中的MEG3比正常鼻咽生物标本中的MEG3大幅减少。携带CC或CA基因型而不是HOTTIPrs1859168AA基因型的患者,有更高的HOTTIP水平,而rs7158663AA或GA基因型患者的MEG3表达明显低于GG基因型携带者。
结论:具有HOTTIPrs1859168和MEG3rs7158663遗传变异的个体可能增加NPC易感性和相关临床病理特征的风险,可能通过影响HOTTIP和MEG3的表达。
OBJECTIVE: Recent studies have indicated that
HOTTIP and MEG3 are associated with the initiation and progression of various types of tumors, including nasopharyngeal carcinoma (NPC). This investigation aimed to elucidate the impact of
HOTTIP and MEG3 polymorphisms on the susceptibility and clinicopathologic characteristics of NPC.
METHODS: This research employed next-generation sequencing and multiplex PCR to assess the polymorphisms of
HOTTIP rs1859168 and MEG3 rs7158663 in 200 NPC and 200 healthy individuals respectively.
HOTTIP and MEG3 expression were assessed via qRT-PCR assessment. Furthermore, the genotypes and alleles frequency of rs1859168 and rs7158663 were compared between healthy and NPC individuals to elucidate their influence on NPC susceptibility and relation with clinicopathologic characteristics.
RESULTS: In comparison with the healthy cohort, the presence of
HOTTIP rs1859168 CC genotype and the C allele were markedly linked with increased NPC incidence (p < 0.05). Furthermore, the MEG3 rs7158663 AA genotype and the A allele also indicated an increased risk of NPC (p < 0.05). The subgroup analysis of age, EBV infection, gender, nationality, smoking, and drinking status revealed no marked association between rs1859168 and rs7158663 genotypes and these potential confounding factors. Moreover, it was observed that rs1859168 CC and rs7158663 AA genotypes were related to local tumor invasion and lymph node metastasis. Additionally, HOTTIP indicated a marked elevation, while MEG3 substantially reduced in NPC samples than the normal nasopharyngeal biospecimens. Patients who carried CC or CA genotypes rather than the HOTTIP rs1859168 AA genotype, had substantially higher HOTTIP levels, while patients with rs7158663 AA or GA genotypes indicated notably lower expression of MEG3 than GG genotype carriers.
CONCLUSIONS: Individuals with genetic variants of HOTTIP rs1859168 and MEG3 rs7158663 might have an increased risk of NPC susceptibility and related clinicopathologic characteristics, potentially by affecting the expression of HOTTIP and MEG3.