Abstract：
HOXA transcript at the distal tip (HOTTIP), a lncRNA, induces cell proliferation and cancer progression. However, the expression and function of HOTTIP in renal cell carcinoma (RCC) were rarely reported. The role of the HOTTIP in RCC was explored in this study. HOTTIP expresses higher in RCC tissues than in normal tissues and indicates poor prognosis based on the TCGA database. The over- and low-expression HOTTIP cell line was established in this research to assess the oncogenic function of HOTTIP in RCC progression. Mechanistic analyses revealed that HOTTIP functioned as a competing endogenous RNA (ceRNA) for miR-506. RIP experiment and luciferase assay were performed to explore the mechanisms of the sponge between HOTTIP and miR-506. HOTTIP down-regulation attenuated cell proliferation, migration, and invasion, which could be rescued by miR-506 down-regulation. On the whole, this study revealed that the HOTTIP/miR-506 axis has a dominant impact on RCC progression and potentially provides a novel strategy for RCC diagnosis and therapy.
摘要：
远端尖端（HOTTIP）的HOXA转录本，lncRNA，诱导细胞增殖和癌症进展。然而,HOTTIP在肾细胞癌（RCC）中的表达和功能很少见报道。本研究探讨了HOTTIP在RCC中的作用。HOTTIP在RCC组织中的表达高于在正常组织中的表达,并且基于TCGA数据库指示不良预后。本研究建立了高表达和低表达HOTTIP细胞系，以评估HOTTIP在RCC进展中的致癌功能。机制分析揭示HOTTIP充当miR-506的竞争性内源性RNA(ceRNA)。进行RIP实验和荧光素酶测定以探讨HOTTIP与miR-506之间的海绵作用机制。HOTTIP下调减弱细胞增殖，迁移,和入侵,这可以通过miR-506下调来拯救。总的来说,这项研究表明,HOTTIP/miR-506轴对RCC进展具有主要影响,并可能为RCC诊断和治疗提供新的策略.
