■运动训练对于预防糖尿病病理性心脏重塑可能是必不可少的。因此,本研究调查了中等强度连续训练(MICT)和高强度间歇训练(HIIT)单独或联合二甲双胍对糖尿病诱发心肌病的影响.
■招募49只Wistar大鼠(雄性)。七组动物治疗6周作为对照,糖尿病,MICT(15m/min,40分钟/天),HIIT(20m/min,40分钟/天),二甲双胍(300mg/kg),HIIT+二甲双胍(Met-HIIT),和MICT+二甲双胍(Met-MICT)。二甲双胍用胃内针头口服给药,用电动跑步机训练运动大鼠(5天/周)。代谢参数,超声心动图指数,组织病理学评估,和评估与心脏纤维化相关的基因表达,肥大,线粒体表现,研究了细胞内钙稳态。
■我们的结果表明,所有干预措施都可以预防糖尿病大鼠的体重减轻,并提高心脏重量/体重比和空腹血糖。两种类型的运动及其二甲双胍组合通过提高心率来改善糖尿病动物的超声心动图指数,分数缩短(FS),射血分数(EF)和左心室收缩末期和舒张末期内径(LVESD和LVEDD)的减少。心钠素(ANP)基因表达,脑钠肽(BNP),转化生长因子(TGF)-β,糖尿病组的胶原蛋白增加。相比之下,过氧化物酶体增殖物激活受体γ辅激活因子1α(PGC-1α)的基因表达,AMP激活蛋白激酶(AMPK),ryanodine受体(RyR),糖尿病动物肌浆网(SERCA)的Ca2ATPase泵减少。单独运动训练或与二甲双胍联合使用可以逆转这些变化。此外,糖尿病诱导的心肌纤维化在治疗组中得到改善.Met-HIIT组比其他组改善糖尿病心肌病的所有指标。
■运动训练,特别是二甲双胍联合用药,减轻糖尿病引起的心脏并发症。运动的有益作用可能与改善病理性心脏重塑和增强心功能有关。
UNASSIGNED: Exercise training could be essential in preventing pathological cardiac remodeling in diabetes. Therefore, the effects of moderate-intensity continuous training (MICT) and high-intensity interval training (
HIIT) singly or plus metformin on diabetes-induced cardiomyopathy were investigated in this study.
UNASSIGNED: Forty-nine Wistar rats (male) were recruited. Seven groups of animals were treated for six weeks as control, diabetes, MICT (15 m/min, 40 min/day),
HIIT (20 m/min, 40 min/day), metformin (300 mg/kg),
HIIT+metformin (Met-
HIIT), and MICT+metformin (Met-MICT). The metformin was orally administered with an intragastrical needle, and the exercised rats were trained (5 days/week) with a motorized treadmill. Metabolic parameters, echocardiographic indices, histopathology evaluation, and assessment of gene expression connected with cardiac fibrosis, hypertrophy, mitochondrial performance, and intracellular calcium homeostasis were investigated.
UNASSIGNED: Our results demonstrated that all the interventions prevented weight loss and enhanced heart weight/body weight ratio and fasting plasma glucose in diabetic rats. Both types of exercise and their metformin combinations improved diabetic animals\' echocardiography indices by enhancing heart rate, fractional shortening (FS), ejection fraction (EF) and reducing end-systolic and end-diastolic diameter of left ventricular (LVESD and LVEDD). Gene expression of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), transforming growth factor (TGF)- β , and collagen increased in the diabetes group. In contrast, the gene expression of peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1 α ), AMP-activated protein kinase (AMPK), ryanodine receptors (RyR), and Ca 2 + ATPase pump of the sarcoplasmic reticulum (SERCA) was reduced in diabetic animals. Exercise training alone or in combination with metformin reversed these changes. Moreover, diabetes-induced cardiac fibrosis was ameliorated in treated groups. All indicators of diabetic cardiomyopathy were improved more in the Met-
HIIT group than in other groups.
UNASSIGNED: Exercise training, notably with metformin combination, alleviated diabetes-induced cardiac complications. The beneficial effects of exercise could be related to improving pathological cardiac remodeling and enhancing cardiac function.