异黄酮囊素-7-O-β-d-吡喃葡萄糖苷(CG)是黄芪(AR)的主要成分,据报道可以抑制体外破骨细胞发育和体内骨丢失。本研究旨在探讨CG对ST2细胞的成骨作用及其机制。结果表明,在成骨分化培养基中,细胞暴露于CG会增加ALP活性,骨钙蛋白(Ocal)mRNA表达与成骨细胞矿化过程。机械上,CG处理增加骨形态发生蛋白2(BMP-2)的表达,p-Smad1/5/8,β-catenin和Runx2,所有这些都是BMP或无翼型MMTV整合位点家族(WNT)/β-catenin信号通路的调节因子。此外,CG的成骨作用被Noggin和DKK-1抑制,它们是BMP和WNT/β-catenin信号通路的经典抑制剂,分别。一起来看,结果表明,CG通过调节BMP/WNT信号通路促进ST2细胞的成骨分化。在此基础上,CG可能是用于改善骨减少疾病的治疗和增强骨再生的有用的先导化合物。
The isoflavone calycosin-7-O-β-d-glucopyranoside (CG) is a principal constituent of Astragalus membranaceus (AR) and has been reported to inhibit osteoclast development in vitro and bone loss in vivo. The aim of this study was to investigate the osteogenic effects of CG and its underlying mechanism in ST2 cells. The results show that exposure of cells to CG in osteogenic differentiation medium increases ALP activity, osteocalcin (Ocal) mRNA expression and the osteoblastic mineralization process. Mechanistically, CG treatment increased the expression of bone morphogenetic protein 2 (BMP-2), p-Smad 1/5/8, β-catenin and Runx2, all of which are regulators of the BMP- or wingless-type MMTV integration site family (WNT)/β-catenin-signaling pathways. Moreover, the osteogenic effects of CG were inhibited by Noggin and DKK-1 which are classical inhibitors of the BMP and WNT/β-catenin-signaling pathways, respectively. Taken together, the results indicate that CG promotes the osteoblastic differentiation of ST2 cells through regulating the BMP/WNT signaling pathways. On this basis, CG may be a useful lead compound for improving the treatment of bone-decreasing diseases and enhancing bone regeneration.