BACKGROUND: This study aimed to assess the quality of various obturation techniques to fill perforation caused by internal root resorption using Micro-computed Tomography.
METHODS: Cone-beam computed tomography images of a maxillary central incisor tooth with perforating internal resorptive defect were used to create a 3D printed model of the affected tooth. The replicas were divided into four groups based on the obturation technique used. The techniques included Group 1: a polydimethylsiloxane-based sealer (GuttaFlow-2) with gutta-percha. Group 2: same as Group 1 except for using a pre-mixed Bioceramic-based sealer (NeoSEALER Flo). Group 3: the defect was filled entirely using the NeoSealer Flo Bioceramic-based sealer. Group 4: the samples were obturated using the warm vertical compaction technique with a resin-based sealer (ADSeal). The resin models were then scanned a micro-computed scanner to evaluate the percentage of voids in each group.
RESULTS: The results showed that NeoSEALER Flo groups had significantly the highest volume of voids while GuttaFlow-2 and warm vertical compaction groups had the lowest void volume.
CONCLUSIONS: GuttaFlow-2 and warm vertical compaction techniques performed best in filling the internal resorptive defect.

UNASSIGNED: This research aimed to assess the push-out bond strength (PBS) to dentin of three distinct obturation materials inside the root canal and identify the failure mechanism.
UNASSIGNED: The research used 30 undamaged human mandibular premolars. The specimens were randomly assigned to three groups, each employing a different sealer (n = 10). Group 1 used AH Plus sealer, Group 2 used GuttaFlow-2 sealer, and Group 3 used bioceramic sealer (CeraSeal). The obturation procedure was performed utilizing the single-cone method with gutta-percha. The specimens were divided into sections and loaded using a universal testing machine. Following PBS testing, every sample underwent a stereomicroscope examination, and the specific failure mechanism was documented.
UNASSIGNED: The average PBS was greatest for AH Plus, followed by CeraSeal and Guttaflow-2. Notable disparities existed between the coronal and apical levels.
UNASSIGNED: AH Plus exhibited superior PBS qualities to root dentin compared to other sealers.

BACKGROUND: There is tendency for unavoidable sealer extrusion in some clinical cases. This might adversely affect host stem cells and affect healing. This study aimed to investigate the effect of different sealers on the cytocompatibility and osteogenic potential of human periodontal ligament stem cells (hPDLSCs).
METHODS: The cytotoxic effect of the extracted elutes of VDW.1Seal (VDW.1), Endosequence BC Sealer HiFlow (ES), GuttaFlow-2 (GF), and ADSeal (AD-S) on the hPDLSCs was determined using the MTT assay. Cell proliferation and migration were assessed by the scratch wound healing assay. Osteogenic differentiation potential was assessed. Measurement of pH values and calcium ions release was performed.
RESULTS: GF had a significantly higher percentage of viable cells. The cell migration assay showed that GF demonstrated the lowest open wound area percentage. GF and AD-S showed the highest calcium nodule deposition. GF demonstrated higher ALP activity than ES. Expression of RUNX2 and OC genes was similar for all sealers, while OPG gene expression was significantly higher for VDW.1 and GF. ES and AD-S displayed the highest pH values on day 1. Calcium ion release of ES and VDW.1 was significantly the highest.
CONCLUSIONS: GuttaFlow-2 and VDW.1Seal sealers have favorable behavior toward host stem cells.