■早期有效的治疗和适当的覆盖对于全层伤口至关重要。羊膜衍生产品最近在组织工程中出现。然而,最佳浓度,控释载体,和处理仍然是挑战。本研究旨在开发和优化原位成形,用于伤口愈合的基于羊膜的水凝胶。
■这里,用甲基丙烯酸酯官能团共轭(GelMA)和角蛋白(wt.1%)改性的明胶水凝胶制备复合基质,负载间充质干细胞(MSCs,1×105细胞/ml)和优化的可溶性羊膜(SAM,0.5mg/ml)。在体外和体内环境中评估最终受试者的物理化学性质。
■体外测定的结果表明,甲基丙烯酰基与明胶的缀合导致形成具有更高机械稳定性的GelMA水凝胶(26.7±1.2kPa)。用硫酸糖胺聚糖(角蛋白)修饰GelMA增加了SAM的控制递送(47.3%vs.84.3%)。通过掺入SAM(0.5mg/ml),封装在水凝胶中的MSC的代谢活性(93%)和增殖(21.2±1.5µg/ml)得到改善。此外,在SAM(0.5mg/ml)/MSC(1×105个细胞/ml)条件培养基的划痕测定中,成纤维细胞的迁移得到促进。GelMA水凝胶组显示,由于高血管生成(6.3±0.3),3周后大鼠的全层皮肤缺损再生,细胞迁移,和上皮化。
结果表明,含有SAM和MSC的原位形成和可调的GelMA水凝胶可用作全厚度伤口再生的有效基质。
UNASSIGNED: Early effective treatment and appropriate coverage are vital for full-thickness wounds. Amnion membrane-derived products have recently emerged in tissue engineering. However, the optimal concentration, carrier for controlled release, and handling have remained challenges. This study aims to develop and optimize an in situ forming, amniotic-based hydrogel for wound healing.
UNASSIGNED: Here, a composite matrix was fabricated with gelatin hydrogel modified with methacrylate functional group conjugated (GelMA) and keratose (wt.1%), loaded with mesenchymal stem cells (MSCs, 1×105 cell/ml) and optimized soluble amniotic membrane (SAM, 0.5 mg/ml). The physicochemical properties of the final subject were evaluated in vitro and in vivo environments.
UNASSIGNED: The results of the in vitro assay demonstrated that conjugation of the methacryloyl group with gelatin resulted in the formation of GelMA hydrogel (26.7±1.2 kPa) with higher mechanical stability. Modification of GelMA with a glycosaminoglycan sulfate (Keratose) increased controlled delivery of SAM (47.3% vs. 84.3%). Metabolic activity (93%) and proliferation (21.2 ± 1.5 µg/ml) of MSCs encapsulated in hydrogel improved by incorporation of SAM (0.5 mg/ml). Furthermore, the migration of fibroblasts was facilitated in the scratched assay by SAM (0.5 mg/ml)/MSCs (1×105 cell/ml) conditioned medium. The GelMA hydrogel groupes revealed regeneration of full-thickness skin defects in rats after 3 weeks due to the high angiogenesis (6.3 ± 0.3), cell migration, and epithelialization.
UNASSIGNED: The results indicated in situ forming and tunable GelMA hydrogels containing SAM and MSCs could be used as efficient substrates for full-thickness wound regeneration.