背景:来自灰树花(GFP)的多糖因其有希望的生物活性和潜在的健康益处而受到全世界的关注。
目的:本研究旨在研究GFP对去卵巢(OVX)小鼠骨质疏松的缓解作用及其机制。
方法:采用OVX法建立绝经后骨质疏松小鼠模型,将48只C57BL/6雌性小鼠随机分为正常组,单独OVX(模型组,n=8),OVX+10mg/kgGFP(GFP-L组,n=8),OVX+20mg/kgGFP(GFP-M组,n=8),OVX+40mg/kgGFP(GFP-H组,n=8),OVX+10mg/kg戊酸雌二醇(阳性组,n=8)。
结果:结果表明,与模型组相比,白细胞介素(IL)-1β的浓度,白细胞介素(IL)-6和肿瘤坏死因子-α(TNF-α)显著降低,超氧化物歧化酶(SOD)和谷胱甘肽(GSH)活性显著升高,髓过氧化物酶(MPO)和丙二醛(MDA)含量显著降低,PINK1,Parkin,Beclin-1和LC3-II在GFP组中显著降低。
结论:这项研究表明,GFP通过减少炎症细胞因子的分泌,减轻卵巢切除诱导的骨质疏松症,体内氧化应激状态的改善,和抑制PINK1/Parkin信号通路。
BACKGROUND: Polysaccharides from
Grifola frondosa(GFP) have gained worldwide attention owing to their promising biological activities and potential health benefits.
OBJECTIVE: This study aimed to investigate the effects of GFP on alleviation of osteoporosis in ovariectomized (OVX) mice and examine the underlying mechanism.
METHODS: A mouse model of postmenopausal osteoporosis was established by OVX method, Forty eight C57BL/6 female mice were randomly divided into Normal group, OVX alone (Model group, n = 8), OVX + 10 mg/kg GFP (GFP-L group, n = 8), OVX + 20 mg/kg GFP (GFP-M group, n = 8), OVX + 40 mg/kg GFP (GFP-H group, n = 8), OVX + 10 mg/kg Estradiol valerate (Positive group, n = 8).
RESULTS: The results showed that compared with Model group, the concentrations of interleukin (IL)-1β, interleukin (IL)-6 and Tumor necrosis factor-α (TNF-α) were significantly reduced, the activity of superoxide dismutase (SOD) and glutathione (GSH) were significantly increased, the content of myeloperoxidase (MPO) and malondialdehyde (MDA) were significantly reduced, and the proteins levels of PINK1, Parkin, Beclin-1 and LC3-II were significantly decreased in the GFP groups.
CONCLUSIONS: This study demonstrates that GFP alleviates ovariectomy-induced osteoporosis via reduced secretion of inflammatory cytokines, improvement in the oxidative stress status in the body, and inhibition of the PINK1/Parkin signaling pathway.