本研究旨在探索增长绩效的变化,糖脂代谢紊乱,肠粘膜屏障,盲肠微生物群落,慢性皮质酮(CORT)诱导的应激(CCIS)肉鸡模型中的代谢产物。结果表明,与对照(CON)肉鸡相比,在CCIS肉鸡中:(I)最终体重(BW),BW增益,平均日增重显著降低。(ii)观察到糖脂代谢紊乱,肠道免疫屏障和物理屏障功能受损。(iii)盲肠微生物多样性和丰富度明显增加。从门到属水平,Firmicutes和Faecalibacterium的丰度显着下降,而丰富的变形杆菌,RuminococcaceaUCG-005和大肠杆菌(志贺氏菌)均显着增加。微生物网络分析和功能通路预测表明,盲肠微生物主要集中在翻译,新陈代谢,核苷酸代谢,和内分泌系统。(四)确定的主要差异代谢物包括类固醇及其衍生物,氨基酸,脂肪酸,和碳水化合物;其中37种代谢物显著上调,而27种代谢物显著下调。这些差异代谢物主要富集在与类固醇激素生物合成和酪氨酸代谢相关的途径中。(v)盲肠菌群与糖脂代谢指标的相关性显示,BW和总胆固醇(TC)与Christensellaceae_R.7_组和志贺氏菌呈正相关,分别。此外,下调的Faecalibacterium和Christensenellaceae与上调的差异表达的代谢物呈负相关。这些结果表明,CCIS改变了盲肠微生物群组成和代谢产物,导致糖脂代谢紊乱,营养代谢和免疫稳态受损,为消除慢性应激对人类健康和动物生产的危害提供理论依据。
目的:本研究旨在确定肠粘膜屏障改变的影响,盲肠菌群群落,和代谢产物的抗生长性能,慢性皮质酮(CORT)诱导的应激(CCIS)肉鸡糖脂代谢紊乱。与对照(CON)肉鸡相比,在CCIS肉鸡中:(I)抗生长性能,糖脂代谢紊乱,观察肠道免疫屏障和物理屏障功能受损。(二)从门到属的水平,Firmicutes和Faecalibacterium的丰度下降;然而,变形杆菌的丰度,RuminococcaceaUCG-005和大肠杆菌(志贺氏菌)增加。(iii)盲肠中的差异代谢产物主要富集在类固醇激素的生物合成和酪氨酸代谢中。(iv)体重(BW)和总胆固醇(TC)与Christensenellaceae_R.7_组和大肠杆菌志贺氏菌呈正相关,分别,而下调的Faecalibacterium和Christensenellaceae与上调的代谢产物呈负相关。我们的发现表明,CCIS通过改变盲肠菌群和代谢产物诱导抗生长性能和糖脂代谢紊乱,为消除慢性应激对人类健康和动物生产的影响提供理论依据。
This study aimed to explore alterations in growth performance, glycolipid metabolism disorders, intestinal mucosal barrier, cecal microbiota community, and metabolites in a chronic corticosterone (CORT)-induced stress (CCIS) broiler model. Results showed that compared with control (CON) broilers, in CCIS broilers: (i) the final body weight (BW), BW gain, and average daily gain were significantly reduced. (ii) The glycolipid metabolism disorder and impairement of intestinal immune barrier and physical barrier function were observed. (iii) Diversity and richness of cecal microbiota were obviously increased. From phylum to genus level, the abundances of Firmicutes and Faecalibacterium were significantly decreased, while the abundances of Proteobacteria, RuminococcaceaeUCG-005, and Escherichia coli (Shigella) were significantly increased. Microbial network analysis and function pathways prediction showed that cecal microbiota was mainly concentrated in translation, metabolism, nucleotide metabolism, and endocrine system. (iv) The main differential metabolites identified include steroids and their derivatives, amino acids, fatty acids, and carbohydrates; among which 37 metabolites were significantly upregulated, while 27 metabolites were significantly downregulated. These differential metabolites were mainly enriched in pathways related to steroid hormone biosynthesis and tyrosine metabolism. (v) Correlation between cecal microbiota and glycolipid metabolism indexes showed that BW and total cholesterol (TC) were positively correlated with Christensenellaceae_R.7_group and Escherichia_Shigella, respectively. Furthermore, the downregulated Faecalibacterium and Christensenellaceae were negatively correlated with the upregulated differentially expressed metabolites. These findings suggested that CCIS altered cecal microbiota composition and metabolites, which led to glycolipid metabolism disorder and impaired the nutritional metabolism and immune homeostasis, providing a theoretical basis for efforts to eliminate the harm of chronic stress to human health and animal production.
OBJECTIVE: The study aimed to determine the influence of altered intestinal mucosal barrier, cecum flora community, and metabolites on anti-growth performance, glycolipid metabolism disorders of chronic corticosterone (CORT)-induced stress (CCIS) broilers. Compared with control (CON) broilers, in CCIS broilers: (i) anti-growth performance, glycolipid metabolism disorder, and impaired intestinal immune barrier and physical barrier function were observed. (ii) From phylum to genus level, the abundances of Firmicutes and Faecalibacterium were decreased; whereas, the abundances of Proteobacteria, RuminococcaceaeUCG-005, and Escherichia coli (Shigella) were increased. (iii) Differential metabolites in cecum were mainly enriched in steroid hormone biosynthesis and tyrosine metabolism. (iv) Body weight (BW) and total cholesterol (TC) were positively correlated with Christensenellaceae_R.7_group and Escherichia_Shigella, respectively, while downregulated Faecalibacterium and Christensenellaceae were negatively correlated with upregulated metabolites. Our findings suggest that CCIS induces anti-growth performance and glycolipid metabolism disorder by altering cecum flora and metabolites, providing a theoretical basis for efforts to eliminate the effect of chronic stress on human health and animal production.