从作物副产品中回收生物活性化合物导致了一种新的废物再利用方式,作为循环经济的一部分。本研究旨在研究洋蓟和花椰菜副产品的详尽代谢物特征(叶子,茎,和花椰菜的小花)作为其价值化和未来实现的先决条件。使用基于NMR的代谢组学方法分析了副产物的水性和有机提取物的代谢谱。游离氨基酸,有机酸,糖,糖多元醇多酚,胺,芥子油苷,脂肪酸,磷酸和半乳糖脂,固醇,和倍半萜内酯的鉴定和定量。特别是,地球朝鲜蓟副产品是健康有益化合物的来源,包括chiro-肌醇(高达10.1毫克/克),鲨肌醇(最高1.8mg/g),倍半萜内酯(cynaropicrin,grosheimin,去氢青蒿素,总计高达45.5mg/g),inulins,和绿原酸(高达7.5毫克/克),而花椰菜副产物包裹生物活性含硫化合物S-甲基-L-半胱氨酸S-氧化物(甲基,高达20.7mg/g)和芥子油苷。根据作物类型,观察到所有代谢物的可变含量(朝鲜蓟与花椰菜)和植物部分(叶子与茎)。这里报告的结果可能会以不同的方式使用,包括新的植物生物兴奋剂和食品补充剂的配方。
The recovery of bioactive compounds from crop byproducts leads to a new perspective way of waste reutilization as a part of the circular economy. The present study aimed at an exhaustive metabolite profile characterization of globe artichoke and cauliflower byproducts (leaves, stalks, and florets for cauliflower only) as a prerequisite for their valorization and future implementations. The metabolite profile of aqueous and organic extracts of byproducts was analyzed using the NMR-based metabolomics approach. Free amino acids, organic acids, sugars, polyols, polyphenols, amines, glucosinolates, fatty acids, phospho- and galactolipids, sterols, and sesquiterpene lactones were identified and quantified. In particular, globe artichoke byproducts are a source of health-beneficial compounds including chiro-inositol (up to 10.1 mg/g), scyllo-inositol (up to 1.8 mg/g), sesquiterpene lactones (cynaropicrin, grosheimin, dehydrocynaropicrin, up to 45.5 mg/g in total), inulins, and chlorogenic acid (up to 7.5 mg/g), whereas cauliflower byproducts enclose bioactive sulfur-containing compounds S-methyl-L-cysteine S-oxide (methiin, up to 20.7 mg/g) and glucosinolates. A variable content of all metabolites was observed depending on the crop type (globe artichoke vs. cauliflower) and the plant part (leaves vs. stalks). The results here reported can be potentially used in different ways, including the formulation of new plant biostimulants and food supplements.