鱼ill不仅是呼吸器官,但对离子调节也至关重要,酸碱控制,排毒,废物排泄和宿主防御。在养殖的大西洋鲑鱼中,多因素g病很常见,仍然知之甚少。了解ill病理生理学至关重要,但是应该避免为此目的而牺牲大量实验动物。因此,体外模型,如细胞系,迫切需要取代鱼类试验。大西洋鲑鱼g上皮细胞系,ASG-10于2018年在挪威兽医学院成立。该细胞系形成表达细胞角蛋白的单层,e-cadherin和桥粒,功能性上皮屏障的标志。为了确定ASG-10用于g功能比较研究的价值,通过进行功能测定并将细胞蛋白质组和转录组与幼年淡水大西洋鲑鱼的g进行比较,将ASG-10的表征更进一步。ASG-10细胞系似乎是由上皮细胞组成的同质细胞系,表达紧密连接蛋白。我们证明了ASG-10形成了一个屏障,单独和与大西洋鲑鱼g成纤维细胞细胞系ASG-13共培养。ASG-10细胞可以吞噬并表达几种ATP结合盒转运蛋白。此外,ASG-10表达参与异源生物生物转化和免疫应答的基因。一起来看,这项研究概述了可以使用ASG-10进行研究的功能,这将对大西洋鲑鱼的体外g上皮研究做出重要贡献。
Fish gills are not only the respiratory organ, but also essential for ion-regulation, acid-base control, detoxification, waste excretion and host defense. Multifactorial gill diseases are common in farmed Atlantic salmon, and still poorly understood. Understanding gill pathophysiology is of paramount importance, but the sacrifice of large numbers of experimental animals for this purpose should be avoided. Therefore, in vitro models, such as cell lines, are urgently required to replace fish trials. An Atlantic salmon gill epithelial cell line, ASG-10, was established at the Norwegian Veterinary institute in 2018. This cell line forms a monolayer expressing cytokeratin, e-cadherin and desmosomes, hallmarks of a functional epithelial barrier. To determine the value of ASG-10 for comparative studies of gill functions, the characterization of ASG-10 was taken one step further by performing functional assays and comparing the cell proteome and transcriptome with those of gills from juvenile freshwater Atlantic salmon. The ASG-10 cell line appear to be a homogenous cell line consisting of epithelial cells, which express tight junction proteins. We demonstrated that ASG-10 forms a barrier, both alone and in co-culture with the Atlantic salmon gill fibroblast cell line ASG-13. ASG-10 cells can phagocytose and express several ATP-binding cassette transport proteins. Additionally, ASG-10 expresses genes involved in biotransformation of xenobiotics and immune responses. Taken together, this study provides an overview of functions that can be studied using ASG-10, which will be an important contribution to in vitro gill epithelial research of Atlantic salmon.