Abstract：
Here we evaluated the gill epithelial cell line ASG-10 from Atlantic salmon, as an in vitro model for research on known water quality challenges in aquaculture. Ammonia/ammonium (NH3/NH4+), a recognized challenge in water-intensive recirculating aquaculture systems (RAS), induced lysosomal vacuolization, reduced protein degradation and cell migration of the ASG-10 cells. Aluminium (Aln+), another challenge in freshwater aquaculture facilities had only minor effects. Next, we investigated the tolerance for direct water exposure of ASG-10. The cells tolerated water with osmolarity between 169 and 419 mOsmol/kg for 24 h. However, cells exposed for 3 h to water at 863 mOsmol/kg changed cellular morphology and induced gene expression related to stress (gpx1, casp3, hsp70), and after 24 h exposure cellular viability was severely reduced. Nevertheless, when the cells were grown in transwell inserts, they tolerated 863 mOsmol/kg for 3 h and induction of stress response associated genes was considerably reduced. Lastly, the ASG-10 cells were exposed to water samples, with no known quality issues, from different aquaculture facilities. The cells showed no differences in viability or morphology compared to their representative control. In conclusion, the ASG-10 cell line is a promising in vitro model to study water quality challenges and whole water samples.
摘要：
在这里，我们评估了来自大西洋鲑鱼的g上皮细胞系ASG-10，作为水产养殖中已知水质挑战研究的体外模型。氨/铵（NH3/NH4+），水密集型循环水产养殖系统(RAS)中公认的挑战，诱导溶酶体空泡化，减少ASG-10细胞的蛋白质降解和细胞迁移。铝(Aln+),淡水水产养殖设施的另一个挑战只有很小的影响。接下来,我们调查了ASG-10对直接水暴露的耐受性。细胞耐受渗透压在169和419mOsmol/kg之间的水24小时。然而，暴露于863mOsmol/kg水中3小时的细胞改变了细胞形态并诱导了与胁迫相关的基因表达（gpx1，casp3，hsp70），暴露24小时后，细胞活力严重降低。然而，当细胞在transwell插入物中生长时，它们耐受863mOsmol/kg3小时，应激反应相关基因的诱导大大降低。最后,将ASG-10细胞暴露于水样中，没有已知的质量问题，来自不同的水产养殖设施。与其代表性对照相比,细胞在活力或形态上没有差异。总之,ASG-10细胞系是研究水质挑战和整个水样的有前途的体外模型。
