■研究表明,心血管健康(CVH)与抑郁症有关。我们旨在使用全血转录组识别与抑郁症状和心血管健康指标共同相关的基因网络。
■我们分析了人类血液转录组数据,以确定基因共表达网络,称为基因模块,贝克的抑郁症清单(BDI-II)评分和心血管健康(CVH)指标作为抑郁症和心血管健康的标志,分别。BDI-II评分来自贝克的抑郁量表,一项21项自我报告清单,用于衡量抑郁症的特征和症状。CVH指标是根据美国心脏协会的标准使用七个指标定义的:吸烟,饮食,身体活动,体重指数(BMI),血压,总胆固醇,和空腹血糖。模块的联合协会,用加权共表达分析确定,以及具有抑郁和CVH标记的模块的成员基因用多变量方差分析(MANOVA)进行测试.
■我们鉴定了具有256个基因的基因模块,这些基因与BDI-II评分和CVH指标均显著相关。根据年龄和性别调整后的MANOVA测试结果,该模块与抑郁和CVH标志物相关.模块中三个最显著的成员基因是YOD1、RBX1和LEPR。模块中的基因富含涉及脑疾病如阿尔茨海默氏症的生物通路,帕金森,和亨廷顿的。
■鉴定的基因模块及其成员可以为抑郁症和CVH提供新的联合生物标志物。
UNASSIGNED: Studies have shown that cardiovascular health (CVH) is related to depression. We aimed to identify gene networks jointly associated with depressive symptoms and cardiovascular health metrics using the whole blood transcriptome.
UNASSIGNED: We analyzed human blood transcriptomic data to identify gene co-expression networks, termed gene modules, shared by Beck\'s depression inventory (BDI-II) scores and cardiovascular health (CVH) metrics as markers of depression and cardiovascular health, respectively. The BDI-II scores were derived from Beck\'s Depression Inventory, a 21-item self-report inventory that measures the characteristics and symptoms of depression. CVH metrics were defined according to the American Heart Association criteria using seven indices: smoking, diet, physical activity, body mass index (BMI), blood pressure, total cholesterol, and fasting glucose. Joint association of the modules, identified with weighted co-expression analysis, as well as the member genes of the modules with the markers of depression and CVH were tested with multivariate analysis of variance (MANOVA).
UNASSIGNED: We identified a gene module with 256 genes that were significantly correlated with both the BDI-II score and CVH metrics. Based on the MANOVA test results adjusted for age and sex, the module was associated with both depression and CVH markers. The three most significant member genes in the module were YOD1, RBX1, and LEPR. Genes in the module were enriched with biological pathways involved in brain diseases such as Alzheimer\'s, Parkinson\'s, and Huntington\'s.
UNASSIGNED: The identified gene module and its members can provide new joint biomarkers for depression and CVH.