PDF(Pubmed)
Abstract：
BACKGROUND: Soybean establishes a mutualistic interaction with nitrogen-fixing rhizobacteria, acquiring most of its nitrogen requirements through symbiotic nitrogen fixation. This crop is susceptible to water deficit; evidence suggests that its nodulation status-whether it is nodulated or not-can influence how it responds to water deficit. The translational control step of gene expression has proven relevant in plants subjected to water deficit.
RESULTS: Here, we analyzed soybean roots\' differential responses to water deficit at transcriptional, translational, and mixed (transcriptional + translational) levels. Thus, the transcriptome and translatome of four combined-treated soybean roots were analyzed. We found hormone metabolism-related genes among the differentially expressed genes (DEGs) at the translatome level in nodulated and water-restricted plants. Also, weighted gene co-expression network analysis followed by differential expression analysis identified gene modules associated with nodulation and water deficit conditions. Protein-protein interaction network analysis was performed for subsets of mixed DEGs of the modules associated with the plant responses to nodulation, water deficit, or their combination.
CONCLUSIONS: Our research reveals that the stand-out processes and pathways in the before-mentioned plant responses partially differ; terms related to glutathione metabolism and hormone signal transduction (2 C protein phosphatases) were associated with the response to water deficit, terms related to transmembrane transport, response to abscisic acid, pigment metabolic process were associated with the response to nodulation plus water deficit. Still, two processes were common: galactose metabolism and branched-chain amino acid catabolism. A comprehensive analysis of these processes could lead to identifying new sources of tolerance to drought in soybean.
RESULTS: Here, we analyzed soybean roots\' differential responses to water deficit at transcriptional, translational, and mixed (transcriptional + translational) levels. Thus, the transcriptome and translatome of four combined-treated soybean roots were analyzed. We found hormone metabolism-related genes among the differentially expressed genes (DEGs) at the translatome level in nodulated and water-restricted plants. Also, weighted gene co-expression network analysis followed by differential expression analysis identified gene modules associated with nodulation and water deficit conditions. Protein-protein interaction network analysis was performed for subsets of mixed DEGs of the modules associated with the plant responses to nodulation, water deficit, or their combination.
CONCLUSIONS: Our research reveals that the stand-out processes and pathways in the before-mentioned plant responses partially differ; terms related to glutathione metabolism and hormone signal transduction (2 C protein phosphatases) were associated with the response to water deficit, terms related to transmembrane transport, response to abscisic acid, pigment metabolic process were associated with the response to nodulation plus water deficit. Still, two processes were common: galactose metabolism and branched-chain amino acid catabolism. A comprehensive analysis of these processes could lead to identifying new sources of tolerance to drought in soybean.
摘要：
背景：大豆与固氮根际细菌建立了相互作用，通过共生固氮获得大部分氮需求。这种作物容易缺水；有证据表明，它的结瘤状态-无论是否结瘤-都会影响它对缺水的反应。已证明基因表达的翻译控制步骤与遭受水分亏缺的植物有关。
结果：这里，我们分析了大豆根在转录时对水分亏缺的差异反应，翻译,和混合（转录+翻译）水平。因此，分析了四个联合处理的大豆根的转录组和翻译组。我们在结瘤和水分限制植物的翻译体水平上的差异表达基因（DEG）中发现了激素代谢相关基因。此外，加权基因共表达网络分析，然后进行差异表达分析，确定了与结瘤和缺水条件相关的基因模块。对与植物对结瘤反应相关的模块的混合DEG子集进行了蛋白质-蛋白质相互作用网络分析，缺水,或他们的组合。
结论：我们的研究表明，上述植物反应中的突出过程和途径部分不同；与谷胱甘肽代谢和激素信号转导有关的术语（2C蛋白磷酸酶）与水分亏缺的反应有关，与跨膜运输相关的术语，对脱落酸的反应，色素代谢过程与结瘤和水分亏缺的反应有关。尽管如此，两个过程是常见的：半乳糖代谢和支链氨基酸分解代谢。对这些过程的全面分析可能会导致确定大豆耐旱性的新来源。
结果：这里，我们分析了大豆根在转录时对水分亏缺的差异反应，翻译,和混合（转录+翻译）水平。因此，分析了四个联合处理的大豆根的转录组和翻译组。我们在结瘤和水分限制植物的翻译体水平上的差异表达基因（DEG）中发现了激素代谢相关基因。此外，加权基因共表达网络分析，然后进行差异表达分析，确定了与结瘤和缺水条件相关的基因模块。对与植物对结瘤反应相关的模块的混合DEG子集进行了蛋白质-蛋白质相互作用网络分析，缺水,或他们的组合。
结论：我们的研究表明，上述植物反应中的突出过程和途径部分不同；与谷胱甘肽代谢和激素信号转导有关的术语（2C蛋白磷酸酶）与水分亏缺的反应有关，与跨膜运输相关的术语，对脱落酸的反应，色素代谢过程与结瘤和水分亏缺的反应有关。尽管如此，两个过程是常见的：半乳糖代谢和支链氨基酸分解代谢。对这些过程的全面分析可能会导致确定大豆耐旱性的新来源。
