■目前,幽门螺杆菌(H.幽门螺杆菌)和胃肠道疾病仍在讨论中。这项研究调查了幽门螺杆菌中不同毒力因子与不同胃肠道疾病之间的关系。
■胃活检标本取自中国160例不同胃肠道疾病患者,包括77例慢性胃炎(CG),36患有消化性溃疡(PUD),38例胃癌(GC)。通过聚合酶链反应(PCR)确定某些毒力基因的存在,并使用卡方检验对结果进行了分析。
■从胃活检标本中成功分离出160株幽门螺杆菌。总的来说,幽门螺杆菌的所有菌株都是cagA,cagE阳性,最常见的vacA基因型为s1(98.8%)和m2(68.1%)。jhp0562、jhp0563、homA、homB,hopQI,HopQII基因占99.4%,32.5%,33.1%,71.3%,100%,和6.9%,分别。这些基因与不同疾病类型之间没有显著关联。在83.1%的菌株中检测到显性hpyIIIR阳性基因型,使其明显比hrgA阳性基因型更普遍(P<0.001)。令人惊讶的是,hrgA和hpyIIIR混合基因型常见,占41.3%。hrgA阳性菌株在GC患者中更为常见(71.1%),与CG患者相比(50.7%,P<0.05)。混合基因型普遍存在,分别占GC和CG患者的55.3%和31.2%。分别。多因素分析显示,hrgA基因与GC呈正相关,增加GC风险[比值比(OR)=3.606,P<0.05]。相比之下,hrgA的存在与CG呈负相关(OR=0.499,P<0.05)。
■这些结果表明,cagA的普遍存在,cagE,vacAs1,jhp0562,homB,并且hopQI使得无法检查疾病特异性与任何这些毒力因子的关联。此外,它们可能协同作用导致中国更多的毒株和严重的疾病。此外,hrgA基因与进展为GC之间有很强的关联,表明其他毒力因子在临床检测中的潜在应用。
UNASSIGNED: At present, the relationship between virulence factors of Helicobacter pylori (H. pylori) and gastrointestinal diseases is still under discussion. This study investigated the association between distinct virulence factors in H. pylori and different gastrointestinal diseases.
UNASSIGNED: Gastric biopsy specimens were obtained from 160 patients with different gastrointestinal diseases in China, including 77 patients with chronic gastritis (CG), 36 with peptic ulcer disease (PUD), and 38 with gastric carcinoma (GC). The presence of certain virulence genes was determined by polymerase chain reaction (PCR), and the results were analyzed using chi-squared tests.
UNASSIGNED: A total of 160 H. pylori strains were successfully isolated from gastric biopsy specimens. Overall, all strains of H. pylori were cagA, cagE positive, and the most common vacA genotypes were s1 (98.8%) and m2 (68.1%). The positive rates of the jhp0562, jhp0563, homA, homB, hopQI, and hopQII genes were 99.4%, 32.5%, 33.1%, 71.3%, 100%, and 6.9%, respectively. There was no significant association between these genes and different disease types. The dominant hpyIIIR-positive genotype was detected in 83.1% of the strains, making it significantly more prevalent than the hrgA-positive genotype (P<0.001). Surprisingly, the mixed genotype of hrgA and hpyIIIR was common and accounted for 41.3%. The hrgA-positive strains were more common in GC patients (71.1%) compared to CG patients (50.7%, P<0.05). The mixed genotype was prevalent and accounted for 55.3% and 31.2% of strains from GC and CG patients, respectively. Multivariate analysis showed that the hrgA gene exhibited a positive correlation with GC and increased the risk of GC [odds ratio (OR) =3.606, P<0.05]. In contrast, the presence of hrgA exhibited a negative correlation with CG (OR =0.499, P<0.05).
UNASSIGNED: These results suggested that the universal presence of cagA, cagE, vacA s1, jhp0562, homB, and hopQI made it impossible to examine disease-specific associations with any of these virulence factors. In addition, they may synergistically contribute to more virulent strains and severe diseases in China. Furthermore, there was a strong association between the hrgA gene and progression to GC, indicating the potential application of other virulence factors in clinical detection.