耐碳青霉烯类鲍曼不动杆菌(CRAB)近年来已成为新的威胁,由于其对抗生素的耐药性迅速增加,因此需要新的有效疗法来对抗这种病原体。噬菌体疗法被认为是治疗CRAB感染的最有希望的替代方法。在这项研究中,一种新的噬菌体,Ab_WF01,可以溶解临床CRAB,是从医院污水中分离出来的。感染的多样性,形态学,一步增长曲线,稳定性,灵敏度,还研究了噬菌体的裂解活性。噬菌体Ab_WF01的基因组大小为41,317bp,GC含量为39.12%,编码51个开放阅读框(ORF)。tRNA,毒力,噬菌体基因组中未检测到抗生素抗性基因。比较基因组和系统发育分析表明,噬菌体Ab_WF01是Friunavirus属的新物种,贝耶林克韦林亚科,和自拟病毒科。体内实验结果表明,噬菌体Ab_WF01显著提高了CRAB感染的海绵状球藻(48h从0%到70%)和小鼠(7天从0%到60%)的存活率。此外,感染后第3天,噬菌体Ab_WF01减少炎症反应,在受感染的组织器官(肺,肝脏,和脾脏)在小鼠CRAB感染模型中。一起来看,这些结果表明,噬菌体Ab_WF01作为潜在的替代药物具有很好的抗CRAB感染的稳定性。
Carbapenem-resistant Acinetobacter baumannii (CRAB) has become a new threat in recent years, owing to its rapidly increasing resistance to antibiotics and new effective therapies are needed to combat this pathogen. Phage therapy is considered to be the most promising alternative for treating CRAB infections. In this study, a novel phage, Ab_WF01, which can lyse clinical CRAB, was isolated and characterized from hospital sewage. The multiplicity of infection, morphology, one-step growth curve, stability, sensitivity, and lytic activity of the phage were also investigated. The genome of phage Ab_WF01 was 41, 317 bp in size with a GC content of 39.12% and encoded 51 open reading frames (ORFs). tRNA, virulence, and antibiotic resistance genes were not detected in the phage genome. Comparative genomic and phylogenetic analyses suggest that phage Ab_WF01 is a novel species of the genus Friunavirus, subfamily Beijerinckvirinae, and family Autographiviridae. The in vivo results showed that phage Ab_WF01 significantly increased the survival rate of CRAB-infected Galleria mellonella (from 0% to 70% at 48 h) and mice (from 0% to 60% for 7 days). Moreover, after day 3 post-infection, phage Ab_WF01 reduced inflammatory response, with strongly ameliorated histological damage and bacterial clearance in infected tissue organs (lungs, liver, and spleen) in mouse CRAB infection model. Taken together, these results show that phage Ab_WF01 holds great promise as a potential alternative agent with excellent stability for against CRAB infections.