近年来,基于纳米材料的过氧化物酶模拟物作为酶传感器的开发由于其突出的特性而吸引了相当大的兴趣,包括强大的稳定性,以及对天然酶的成本效益。在这项工作中,通过溶胶-凝胶法首次制备了铜(Cu-MSN)功能化的介孔二氧化硅纳米颗粒作为一种新型人工酶。通过显色过氧化物酶底物的氧化对Cu-MSN的催化活性进行了全面的研究,3,3\',5,5'-四甲基联苯胺(TMB),和(2,2'-氮杂-双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS),在H2O2的存在下。结果表明,所制备的样品的过氧化物酶样活性显著高于其他纳米颗粒。此外,为了研究,开发了一种基于Cu-MSN的类酶活性的简便快速传感方法来检测H2O2和谷胱甘肽(GSH),以检查所提出的生物传感器的效能。初步分析表明,H2O2和GSH的检测限(LOD)为0.2和0.0126μM,在0.9-100和0.042-1μM的范围内,分别。这些发现支持了传感器在检测领域中效率的主张。此外,人血清被用作真实样本以获得更多证据。
In recent years, the development of nanomaterials-based peroxidase mimics as enzyme sensors has been attracting considerable interest due to their outstanding features, including potent stability, and cost-effectiveness toward natural enzymes. In this work, mesoporous silica nanoparticles functionalized by copper (Cu-MSN) were prepared as a new artificial enzyme for the first time through the sol-gel procedure. A comprehensive investigation of the catalytic activity of Cu-MSN was done through the oxidation of chromogenic peroxidase substrates, 3,3\',5,5\'-tetramethylbenzidine (TMB), and (2,2\'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), in the presence of H2O2. The results indicate that the peroxidase-like activity of the as-prepared sample is significantly higher than other nanoparticles. Additionally, for the study, a facile and rapid sensing method based on the enzyme-like activity of Cu-MSN to detect H2O2 and glutathione (GSH) was developed to examine the potency of the proposed biosensor. Preliminary analysis revealed that the limit of detection (LOD) of H2O2 and GSH is 0.2 and 0.0126 μM, in the range of 0.9-100 and 0.042-1 μM, respectively. These findings support the claims for the efficiency of the sensor in detection fields. Also, human serum was utilized as the real sample to obtain additional evidence.