未经证实:有必要调查植物化学物质与手术切除是否可以作为TT患者更好的管理选择,而不是单独的手术切除(SD)。
UNASSIGNED:本研究的描述性横断面部分是基于问卷调查的,涉及参与者的社会人口统计学特征及其在TT管理方面的经验。在实验部分,雄性大鼠(n=32)分为:假,缺血再灌注损伤(IRI),二氯甲烷(DCM)和乙醇级分(100mg/kg)的CO。组织GPx的评价,总硫醇,SOD,完成MDA和H2O2。亚硝酸盐的血清估计,TNF-α和IL-6,MPO,精子运动性,还进行了计数和活力。评估bax和caspase3的组织表达。
UNASSIGNED:68.9%的受访者认为单独的SD在TT管理中无效,而83.6%的受访者表示需要通过药物来增加手术。IRI增加的氧化应激标志物如H2O2、MDA和亚硝酸盐在治疗后组降低,随着GSH的组织水平显着增加,GST,SOD,GPx,和总硫醇。IRI中炎症介质升高,而治疗后大鼠显示显著降低。IRI显著降低精子数量,这通过后处理而逆转。在IRI大鼠中Bax和caspase3增加,而CO组分后处理减少了它们。
UNASSIGNED:通过临床医生的经验,定量横断面研究表明,单靠手术治疗TT并不有效。用CO叶部分增强处理通过抑制促凋亡蛋白表达来抑制睾丸IRI,氧化应激和炎症。
UNASSIGNED: There is need to investigate whether phytochemicals along with surgical detorsion could serve as better managements options in TT patients rather than surgical detorsion (SD) alone.
UNASSIGNED: The descriptive cross-sectional part of this study is questionnaire-based addressing sociodemographic characteristics of participants and their experience in management of TT. In the experimental part, male rats (n = 32) were grouped into: sham, Ischemia-reperfusion injury (IRI), dichloromethane (DCM) and ethanol fraction (100 mg/kg) of CO. Evaluation of tissue GPx, total thiol, SOD, MDA and H2O2 was done. Serum estimations of nitrite, TNF-α and IL-6, MPO, sperm motility, count and viability was also carried out. Tissue expression of bax and caspase 3 was assessed.
UNASSIGNED: 68.9 % respondents agreed that SD alone is non-effective in the management of TT while 83.6 % reported a need to augment surgery with medications. Oxidative stress markers like H2O2, MDA and nitrite increased by IRI were decreased in post-treatment groups, along with a significant increase in the tissue level of GSH, GST, SOD, GPx, and total thiol. Inflammatory mediators were elevated in IRI while post-treatment rats showed significant decrease. IRI decreased sperm count significantly this was reversed by post-treatment. Bax and caspase 3 was increased in IRI rats and post-treatment with CO fractions reduced them.
UNASSIGNED: Quantitative cross-sectional study has revealed through experience of clinicians that surgical detorsion alone is not effective in managing TT. Augmented treatment with CO leaf fractions suppressed testicular IRI through inhibition of pro-apoptotic proteins expression, oxidative stress and inflammation.