本甲虫是农田生态系统害虫的主要天然捕食者,是转基因作物环境安全评价的重要非目标指示昆虫。转基因作物的商业化种植可能会导致P.japonica经常暴露于Bt蛋白环境。在这项研究中,生物学特征,酶活性,并研究了Cry1B蛋白处理后日本紫苏的解毒和代谢表达水平。在发育持续时间上没有观察到显著差异,出苗率,与对照组相比,喂食幼虫0.5mg/mL的Cry1B蛋白后不同年龄的体重或体重。此外,谷胱甘肽S-转移酶(GST)的活性没有显着差异,过氧化氢酶(CAT),饲喂0.25mg/mL和0.5mg/mLCry1B蛋白后的过氧化物酶(POD)。然而,当Cry1B蛋白浓度增加到1.0mg/mL时,商品及服务税的活动,CAT,POD明显增加。与对照组相比,大多数解毒代谢相关基因的表达水平没有显着差异;只有少数基因在单个浓度下表达水平发生变化(CYP345B1,CYP4Q2,CYP9F2,GST,和微粒体GST)。总的来说,这些结果表明,Cry1B蛋白对日本紫苏的生物学特性影响很小或没有影响。与酶活性和解毒相关的基因在高浓度刺激下差异表达。因此,这项研究表明Cry1B对捕食者P.japonica的潜在风险可以忽略不计。
The lady beetle Propylea japonica is a dominant natural predator of insect pests in farmland ecosystems and an important non-target indicator insect for the environmental safety assessment of GM crops. The commercial cultivation of GM crops may cause P. japonica to frequently be exposed to the Bt protein environment. In this study, the biological characteristics, enzyme activity, and expression levels of detoxification and metabolism in P. japonica were studied after Cry1B protein treatment. No significant differences were observed in developmental duration, emergence rate, or body weight at different ages after feeding larvae 0.5 mg/mL of Cry1B protein compared with the control. Furthermore, there were no significant differences in the activities of glutathione S-transferase (GST), catalase (CAT), and peroxidase (POD) after feeding 0.25 mg/mL and 0.5 mg/mL Cry1B protein. However, when the concentration of Cry1B protein increased to 1.0 mg/mL, the activities of the GST, CAT, and POD increased significantly. Compared with the control group, there were no significant differences in the expression levels of most detoxification metabolism related genes; only a few genes had changed expression levels at the individual concentrations (CYP345B1, CYP4Q2, CYP9F2, GST, and microsomal GST). Overall, these results suggest that Cry1B protein has little or no effect on the biological characteristics of P. japonica. Genes related to enzyme activity and detoxification are differentially expressed at high concentration stimulation. Therefore, this research suggests that the potential risks of Cry1B for the predator P. japonica are negligible.