■西花地虫(TO)具有许多生物学活性,包括血糖调节。因此,它被用于治疗糖尿病。TO已被证明会导致胰岛素介导的低血糖,导致低血糖后高血糖。然而,低血糖后高血糖的机制仍然知之甚少.
■这项研究旨在确定葡萄糖调节激素和酶对TO治疗的反应。
■35只雄性Wistar大鼠分为7个口服治疗组(n=5/组),7-接受100mg/kg或200mg/kgTO,10或14天。
■使用TO的7天治疗显着增加了胰岛素水平,胰高血糖素,和葡萄糖-6-磷酸酶(G6Pase)活性,但降低了葡萄糖的水平,肾上腺素,和葡萄糖激酶(GCK)活性。用100mg/kg的TO治疗10天增加了葡萄糖并降低了GCK活性,而200mg/kg相同的时间增加了葡萄糖,胰岛素,GCK和G6Pase活性降低,但胰高血糖素降低。用100mg/kgTO治疗14天,降低了葡萄糖和胰高血糖素,但增加了皮质醇,而相同持续时间的200mg/kgTO增加胰岛素,但降低胰高血糖素和GCK活性。
■由于胰高血糖素和G6Pase活性增加,GCK活性降低。此外,胰高血糖素反应主要取决于葡萄糖而不是胰岛素。
UNASSIGNED: Telfairia occidentalis (TO) has many biological activities including blood glucose regulation. Thus, it is being used in the treatment of diabetes mellitus. TO has been shown to cause insulin-mediated hypoglycaemia, which leads to post-hypoglycaemic hyperglycaemia. However, the mechanism involved in the post-hypoglycaemic hyperglycaemia is still poorly understood.
UNASSIGNED: This research was designed to determine the response of glucoregulatory hormones and enzymes to TO treatment.
UNASSIGNED: Thirty-five male Wistar rats were divided into seven oral treatment groups (n = 5/group), which received either of 100 mg/kg or 200 mg/kg TO for 7-, 10- or 14 days.
UNASSIGNED: The 7-day treatment with TO significantly increased the levels of insulin, glucagon, and glucose-6-phosphatase (G6Pase) activity but decreased the levels of glucose, adrenaline, and glucokinase (GCK) activity. The 10-day treatment with 100 mg/kg TO increased glucose and decreased GCK activity while 200 mg/kg for the same duration increased glucose, insulin, GCK and G6Pase activities but reduced glucagon. The 14-day treatment with 100 mg/kg TO decreased glucose and glucagon but increased cortisol, while 200 mg/kg TO for same duration increased insulin, but reduced glucagon and GCK activity.
UNASSIGNED: The TO\'s post-hypoglycaemic hyperglycaemia results from increased glucagon and G6Pase activity, and reduced GCK activity. Moreover, the glucagon response mainly depends on glucose rather than insulin.