本研究研究了福竹砖茶多糖(FBTPs)对环磷酰胺(Cy)诱导的免疫抑制小鼠的免疫保护作用。结果表明,高剂量的FBTP给药通过改善机体特征显著减轻Cy引起的免疫损伤,器官指数,小鼠的免疫反应和氧化应激。进一步的微生物群分析表明,FBTP通过增加几种有益菌乳酸菌,明显恢复了Cy引起的微生物菌群失调。Allobaculum,未分类的_f_幼虫科和norank_f_幼虫科,同时减少拟杆菌,norank_f_Ruminoccaceae,Colidestribacter,Alloprevotella,norank_f_脱硫弧菌科和螺杆菌。同时,代谢组学分析发现,FBTP显著改变了一系列微生物代谢物,包括肌苷,脱氧肌苷,牛磺酸,芥子酸,麦芽三糖,丁酸,溶血磷脂酰胆碱(LysoPCs),溶血磷脂酸(LysoPAs)和胆碱。这些改变的代谢物与嘌呤代谢有关,ABC运输商,硫代谢,神经活性配体-受体相互作用,苯丙素的生物合成,碳水化合物的消化和吸收,蛋白质消化吸收,癌症中的胆碱代谢和甘油磷脂代谢途径,主要与免疫反应有关,免疫抑制小鼠的抗氧化能力和能量供应。此外,在特定微生物群和有效代谢产物之间观察到一些显著的相关性。这些结果为FBTPs在调节肠道微生物群和代谢方面的免疫保护作用提供了新的见解。这有助于深入了解FBTPs的营养,并为进一步利用FBT砖茶(FBT)提供坚实的基础。
The present study investigated the immune-protective effect of polysaccharides from Fuzhuan brick tea (FBTPs) in cyclophosphamide (Cy)-induced immunosuppressive mice. The results showed that high-dose of FBTPs administration remarkably alleviated Cy-evoked immune damage through improving the body features, organ indices, immune responses and oxidative stress in the mice. Further microbiota analysis revealed that FBTPs obviously restored Cy-evoked microbial dysbiosis by increasing several beneficial bacteria Lactobacillus, Allobaculum, Unclassified_f_Lachnospiraceae and norank_f_Lachnospiraceae, while reducing Bacteroides, norank_f_Ruminococcaceae, Colidextribacter, Alloprevotella, norank_f_Desulfovibrionaceae and Helicobacter. Meanwhile, metabolomics analysis found that FBTPs significantly altered a range of microbial metabolites, including inosine, deoxyinosine, taurine, sinapic acid, maltotriose, butyric acid, lysophosphatidyl cholines (LysoPCs), lysophosphatidic acids (LysoPAs) and choline. These altered metabolites were involved in purine metabolism, ABC transporters, sulfur metabolism, neuroactive ligand-receptor interaction, biosynthesis of phenylpropanoids, carbohydrate digestion and absorption, protein digestion and absorption, choline metabolism in cancer and glycerophospholipid metabolism pathways, which were mainly related to immune responses, antioxidant capacity and energy supply of the immunosuppressive mice. Additionally, some significant correlations were observed between the specific microbiota and effective metabolites. These results provide a novel insight into the immune-protective effect of FBTPs on regulating the intestinal microbiota and metabolism, which are helpful for thoroughly understanding the nutrition of FBTPs and providing a solid basis for the deeper utilization of Fuzhuan brick tea (FBT).