背景:小麦是一种重要的谷物作物,受到了镰刀菌的严重威胁。Nup2,核孔复合物的成员,在调节真核生物核蛋白的转运和参与基因调控中起着重要作用。剖析病原真菌中核孔蛋白的功能可能为新型杀菌剂提供有效的靶标。
结果:突变体表现出营养生长缺陷,无性/性发育异常。FgNup2的缺乏导致禾谷镰刀菌对细胞壁破坏物的抗性增加,对金属离子的敏感性增加。致病性分析表明,该突变体对开花小麦穗的毒力显着降低,与观察到的脱氧雪腐镰刀菌烯醇(DON)产量减少一致。此外,我们表明FgNup2与TRI家族的转录因子FgTri6协同作用,调节毒素产生基因的表达,which,反过来,影响DON和相关毒素的生物合成。
结论:这项研究表明,FgNup2在生长发育中起重要作用,细胞壁完整性,应激反应,致病性,和DON的合成。©2024化学工业学会。
BACKGROUND: Wheat is an important grain crop that has been under serious threat from Fusarium graminearum. Nup2, a member of the nuclear pore complex, plays an important role in regulating eukaryotic nuclear protein transport and participates in gene regulation. Dissecting the function of nuclear pore proteins in pathogenic fungi may provide effective targets for novel fungicides.
RESULTS: Mutants exhibited nutritional growth defects, asexual/sexual developmental abnormalities. Deficiency of FgNup2 resulted in increased resistance of Fusarium graminearum to cell wall disruptors and increased sensitivity to metal ions. Pathogenicity analyses showed that the mutant was significantly less virulent on flowering wheat ears, consistent with the observed decrease in deoxynivalenol (DON) production. Furthermore, we showed that FgNup2 interacts synergistically with FgTri6, a transcription factor of the TRI family, to regulate the expression of toxin-producing genes, which, in turn, affects the biosynthesis of DON and related toxins.
CONCLUSIONS: This study revealed that FgNup2 plays important roles in the growth and development, cell wall integrity, stress response, pathogenicity, and DON synthesis of F. graminearum. © 2024 Society of Chemical Industry.