背景:微生物生物质的使用,如真菌生物质,催化甘油三酯(TG)的酯交换为生物柴油生产提供了一种可持续的、经济的替代品,同时仍具有昂贵的固定化酶的主要优点。
结果:黄曲霉和根霉的生物质用于催化废煎炸油(WFO)中TG的酯交换反应。异丙醇作为酰基受体降低了生物质的催化能力,而甲醇是最有效的酰基受体,最终脂肪酸甲酯(FAME)浓度为85.5%和89.7%,w/w,对于Stolonifer和A.flavus,分别。测试了真菌生物质的不同混合物,较高比例的黄曲霉生物质提高了混合物的催化能力。以在合成废水中培养的索氏梭菌为原料培养黄曲霉。产生的生物质具有与对照培养基中产生的生物质相同的催化能力。采用响应面法(RSM),采用中心复合设计(CCD)优化黄花生物质催化酯交换反应,那里的温度,甲醇浓度,选择生物量浓度进行优化。验证了模型的意义,建议的最佳反应条件为25.5°C,以14%的转速搅拌250RPM,w/w,生物量,3mol/L甲醇,和24小时的反应持续时间。测试了建议的最佳条件以验证模型和95.53%的最终FAME浓度。w/w被检测到。
结论:生物质混合物可能是为工业应用提供比固定化酶更便宜的技术解决方案的合理可能性。在从废水处理中回收的微藻上培养的真菌生物质用于酯交换反应的催化,为生物炼制提供了另一个难题。优化酯交换反应导致有效的预测模型,最终FAME浓度为95.53%,w/w。
BACKGROUND: The use of microbial biomasses, such as fungal biomass, to catalyze the transesterification of triglycerides (TG) for biodiesel production provides a sustainable, economical alternative while still having the main advantages of expensive immobilized enzymes.
RESULTS: Biomasses of Aspergillus flavus and Rhizopus stolonifera were used to catalyze the transesterification of TG in waste frying oil (WFO). Isopropanol as an acyl-acceptor reduced the catalytic capability of the biomasses, while methanol was the most potent acyl-acceptor with a final fatty acid methyl ester (FAME) concentration of 85.5 and 89.7%, w/w, for R. stolonifer and A. flavus, respectively. Different mixtures of the fungal biomasses were tested, and higher proportions of A. flavus biomass improved the mixture\'s catalytic capability. C. sorokiniana cultivated in synthetic wastewater was used as feedstock to cultivate A. flavus. The biomass produced had the same catalytic capability as the biomass produced in the control culture medium. Response surface methodology (RSM) was adopted using central composite design (CCD) to optimize the A. flavus biomass catalytic transesterification reaction, where temperature, methanol concentration, and biomass concentration were selected for optimization. The significance of the model was verified, and the suggested optimum reaction conditions were 25.5 °C, 250 RPM agitation with 14%, w/w, biomass, 3 mol/L methanol, and a reaction duration of 24 h. The suggested optimum conditions were tested to validate the model and a final FAME concentration of 95.53%. w/w was detected.
CONCLUSIONS: Biomasses cocktails might be a legitimate possibility to provide a cheaper technical solution for industrial applications than immobilized enzymes. The use of fungal biomass cultivated on the microalgae recovered from wastewater treatment for the catalysis of transesterification reaction provides an additional piece of the puzzle of biorefinery. Optimizing the transesterification reaction led to a valid prediction model with a final FAME concentration of 95.53%, w/w.