Abstract：
The world\'s hunger for novel food ingredients drives the development of safe, sustainable, and nutritious novel food products. For foods containing novel proteins, potential allergenicity of the proteins is a key safety consideration. One such product is a fungal biomass obtained from the fermentation of Rhizomucor pusillus. The annotated whole genome sequence of this strain was subjected to sequence homology searches against the AllergenOnline database (sliding 80-amino acid windows and full sequence searches). In a stepwise manner, proteins were designated as potentially allergenic and were further compared to proteins from commonly consumed foods and from humans. From the sliding 80-mer searches, 356 proteins met the conservative >35% Codex Alimentarius threshold, 72 of which shared ≥50% identity over the full sequence. Although matches were identified between R. pusillus proteins and proteins from allergenic food sources, the matches were limited to minor allergens from these sources, and they shared a greater degree of sequence homology with those from commonly consumed foods and human proteins. Based on the in silico analysis and a literature review for the source organism, the risk of allergenic cross-reactivity of R. pusillus is low.
摘要：
世界对新型食品成分的渴望推动了安全食品的发展，可持续,和营养新颖的食品。对于含有新型蛋白质的食物，蛋白质的潜在变应原性是一个关键的安全性考虑因素。一种这样的产物是从根瘤菌的发酵获得的真菌生物质。对该菌株的注释的全基因组序列进行针对AllergenOnline数据库的序列同源性搜索(滑动80-氨基酸窗口和全序列搜索)。以逐步的方式，蛋白质被指定为潜在的变应原，并进一步比较蛋白质从一般食用的食物和人类。从滑动的80-mer搜索中，356个蛋白质符合保守的>35%的食品法典阈值，其中72个在整个序列上共享≥50%的同一性。尽管鉴定了R.pusillus蛋白和来自过敏性食物来源的蛋白之间的匹配，比赛仅限于来自这些来源的轻微过敏原，它们与通常食用的食物和人类蛋白质的序列同源性更高。基于对源生物的计算机分析和文献综述，R.pusillus过敏交叉反应的风险较低。
