背景:今天,合成化学品在玻璃化溶液中用于冷冻保存研究,以模拟天然冷冻保护剂,为自然界中的生物体提供对冷冻胁迫的耐受性。就植物而言,PVS2,含甘油,二甲基亚砜(Me2SO),乙二醇和蔗糖,被认为是成功冷冻保存的黄金标准。然而,Me2SO通常会对某些植物细胞产生毒性,对冷冻和/或解冻后的生存力产生不利影响。因此,廉价替代(或大幅减少)Me2SO,无毒和天然的冷冻保护剂成为玻璃化溶液的高度优先事项,或减少其含量对植物的冷冻保存越来越重要。Fructans,蔗糖衍生物主要由果糖残基组成,是候选的冷冻保护剂。
目的:受自然界保护作用的启发,我们在这里探索,第一次,8种结构不同的果聚糖作为植物冷冻保存中的冷冻保护剂的潜力。
方法:拟南芥幼苗用作一步玻璃化方法的模型系统。评估了具有不同Me2SO和果聚糖含量的PVS2溶液。
结果:发现分枝低DPgraminan,从乳白色阶段的小麦籽粒中提取,在冷冻保存后,含有12.5%Me2SO的测试果聚糖中回收率最高(85%),这与用含有15%Me2SO的原始PVS2观察到的活力(90%)非常接近。此外,其保护功效可以通过添加维生素C作为抗氧化剂来进一步优化。
结论:这种新型制剂为各种作物的冷冻保存提供了很好的前景。Doi.org/10.54680/fr24410110512。
BACKGROUND: Today, synthetic chemicals are used in vitrification solutions for cryopreservation studies to mimic natural cryoprotectants that supply tolerance to organisms in nature against freezing stress. In the case of plants, PVS2, containing glycerol, dimethyl sulfoxide (Me2SO), ethylene glycol and sucrose, is considered as the golden standard for successful cryopreservation. However, Me2SO can generally cause toxicity to certain plant cells, adversely affecting viability after freezing and/or thawing. Hence, the replacement (or substantial reduction) of Me2SO by cheap, non-toxic and natural cryoprotectants became a matter of high priority to vitrification solutions or reducing their content gained escalating importance for the cryopreservation of plants.
Fructans, sucrose derivatives mainly consisting of fructose residues, are candidate cryoprotectants.
OBJECTIVE: Inspired by their protective role in nature, we here explored, for the first time, the potential of an array of 8 structurally different
fructans as cryoprotectants in plant cryopreservation.
METHODS: Arabidopsis thaliana L. seedlings were used as a model system with a one-step vitrification method. PVS2 solutions with different Me2SO and fructan contents were evaluated.
RESULTS: It was found that branched low DP graminan, extracted from milky stage wheat kernels, led to the highest recovery (85%) among tested
fructans with 12.5% Me2SO after cryopreservation, which was remarkably close to the viability (90%) observed with the original PVS2 containing 15% Me2SO. Moreover, its protective efficacy could be further optimized by addition of vitamin C acting as an antioxidant.
CONCLUSIONS: Such novel formulations offer great perspectives for cryopreservation of various crop species. Doi.org/10.54680/fr24410110512.