Oncorhynchusmykiss,一个重要的水产养殖物种,具有许多生物学和药理学功能的化合物,包括抗氧化剂,抗癌,抗微生物,和抗肥胖作用。然而,从Oncorhynchusmykiss卵中提取的脂质对LPS诱导的RAW264.7细胞的可能抗炎作用尚未阐明。目前的研究确定了从O.mykiss鸡蛋中提取的脂质中的13种脂肪酸,其中含有大量(占总脂肪酸的51.92%)的多不饱和脂肪酸(PUFA),尤其是DHA(33.66%)和EPA(7.77%)。这些O.mykiss脂质(100-400μg/mL)通过抑制LPS刺激的RAW264.7细胞中的NO和iNOS表达而显示出显著的抗炎作用。它们还抑制促炎细胞因子IL-1β的表达,IL-6和TNF-α,同时上调抗炎细胞因子IL-10、IL-11和TGF-β。来自O.mykiss的这些脂质有效地抑制了LPS诱导的CD86作为RAW264.7细胞上的表面生物标志物的表达。此外,O.mykiss脂质抑制p38,JNK的磷酸化,和ERK1/2和磷酸化NF-κB亚基p65的表达。这些发现表明O.mykiss脂质通过抑制NF-κB和MAPK信号通路而具有抗炎特性。
Oncorhynchus mykiss, a significant aquaculture species, possesses compounds with numerous biological and pharmacological functions, including antioxidant, anticancer, anti-microbial, and anti-obesity effects. However, possible anti-inflammatory effects of lipids extracted from O. mykiss eggs on RAW264.7 cells induced by LPS have not been elucidated yet. The current study identified 13 fatty acids in lipids extracted from O. mykiss eggs that contained high amounts (51.92% of total fatty acids) of polyunsaturated fatty acids (PUFAs), especially DHA (33.66%) and EPA (7.77%). These O. mykiss lipids (100-400 μg/mL) showed significant anti-inflammatory effects by inhibiting NO and iNOS expression in LPS-stimulated RAW264.7 cells. They also inhibited expression of pro-inflammatory cytokines IL-1β, IL-6, and TNF-α, while upregulating anti-inflammatory cytokines IL-10, IL-11, and TGF-β. These lipids from O. mykiss effectively inhibited LPS-induced expression CD86 as a surface biomarker on RAW264.7 cells. Additionally, O. mykiss lipids suppressed phosphorylation of p38, JNK, and ERK1/2 and the expression of phosphorylated NF-κB subunit p65. These findings indicate that O. mykiss lipids possess anti-inflammatory properties by inhibiting NF-κB and MAPK signaling pathways.