背景:内窥镜超声引导下细针抽吸活检(EUSFNAB)是一种成熟的成年患者诊断方法,但很少在儿科人群中使用。卢布尔雅那大学临床中心胃肠病学临床学系和病理学研究所细胞病理学系,医学院,卢布尔雅那大学,斯洛文尼亚,自2010年推出以来,一直在EUSFNAB上密切合作。该研究的目的是回顾儿童胰腺肿瘤的EUSFNAB病例。
方法:在病理学研究所(IP)的数字档案中,医学院(FM),卢布尔雅那大学(UL),我们在儿童中发现了6例EUSFNAB,3有胰腺的EUSFNAB,其中2人患有肿瘤的细胞病理学诊断。在第一种情况下,病变为超声检查实性,细胞样本包含分支的乳头状结构,周围是带有核沟的小细胞聚集体。在第二种情况下,病变是超声检查的囊性,主要是坏死,只有单个保存的细胞。通过免疫组织化学染色在两种情况下都发现了β-catenin的阳性核反应。
结果:在这两种情况下,胰腺实性假乳头状瘤的细胞病理学诊断,这些病例代表了2010年以来卢布尔雅那儿童医院胰腺肿瘤的全部儿科病例。在EUSFNAB期间和之后没有不良事件。肿瘤切除标本的组织病理学检查证实了细胞病理学诊断。
结论:我们的经验表明,EUSFNAB是诊断小儿胰腺肿瘤的一种安全有效的方法,正如文献中的发现所支持的那样。
BACKGROUND: Endoscopic ultrasound-guided fine needle aspiration biopsy (EUS FNAB) is a well established diagnostic method in adult patients, but is rarely used in the paediatric population. The Clinical Department of Gastroenterology at the University Clinical Centre Ljubljana and the Department of Cytopathology at the Institute of Pathology, Faculty of Medicine, University of Ljubljana, Slovenia, have been closely collaborating on EUS FNAB since the introduction in 2010. The aim of the study was to review the cases of EUS FNAB of pancreatic neoplasms in children.
METHODS: In the digital archive of the Institute of Pathology (IP), Faculty of Medicine (FM), University of Ljubljana (UL), we found 6 cases of EUS FNAB in children, 3 had EUS FNAB of the pancreas, 2 of whom had a cytopathologic diagnosis of a tumour. In the first case, the lesion was ultrasonographically solid, and the cell sample contained branching papillary structures surrounded by aggregates of small cells with nuclear grooves. In the second case, the lesion was ultrasonographically cystic, and predominantly necrosis was seen, with only single preserved cells. Positive nuclear reaction for β-catenin was found in both cases by immunohistochemical staining.
RESULTS: In both cases, the cytopathological diagnosis of solid pseudopapillary neoplasm of the pancreas was made, the cases represent the totality of paediatric cases of pancreatic neoplasms from the Children\'s Hospital Ljubljana since 2010. There were no adverse events during and after EUS FNAB. A histopathological examination of the tumour resection specimens confirmed the cytopathological diagnosis.
CONCLUSIONS: Our experience indicates that EUS FNAB is a safe and effective method for diagnosing pancreatic neoplasms in the pediatric population, as supported by the findings in the literature.