目标:共聚集,一种高度特异性的细胞-细胞相互作用机制,在多物种生物膜形成中起着关键作用。虽然它主要在口腔环境中进行研究,它在水生系统中的发生也是公认的。考虑到工程水系统中生物膜形成的经济和健康相关影响,了解其机制至关重要。这里,我们假设在蛋白质组水平上可追溯的差异可能决定了共聚集能力.
结果:两株Delftiaacidovorans,从饮用水中分离进行了研究。首先,体外运动试验表明,共聚集菌株(C)比非共聚集菌株(C-)具有更多的蜂群和抽搐运动。通过TEM,我们证实了两种菌株都存在鞭毛。通过蛋白质组学,我们在C+中检测到显著较高的IV型菌毛抽搐运动蛋白表达,符合运动性测定。此外,鞭毛环蛋白在C+中更丰富,而参与鞭毛钩形成的鞭毛钩(FlE和FilG)仅在C-中检测到。所有结合的结果都表明其细胞附件中染色剂之间的结构和构象差异。
结论:本研究提供了一种鉴定蛋白质生物标志物以检测未表征菌株的共聚集能力的替代方法。
OBJECTIVE: Coaggregation, a highly specific cell-cell interaction mechanism, plays a pivotal role in multispecies biofilm formation. While it has been mostly studied in oral environments, its occurrence in aquatic systems is also acknowledged. Considering biofilm formation\'s economic and health-related implications in engineered water systems, it is crucial to understand its mechanisms. Here, we hypothesized that traceable differences at the proteome level might determine coaggregation ability.
RESULTS: Two strains of Delftia acidovorans, isolated from drinking water were studied. First, in vitro motility assays indicated more swarming and twitching motility for the coaggregating strain (C+) than non-coaggregating strain (C-). By transmission electronic microscopy, we confirmed the presence of flagella for both strains. By proteomics, we detected a significantly higher expression of type IV pilus twitching motility proteins in C+, in line with the motility assays. Moreover, flagellum ring proteins were more abundant in C+, while those involved in the formation of the flagellar hook (FlE and FilG) were only detected in C-. All the results combined suggested structural and conformational differences between stains in their cell appendages.
CONCLUSIONS: This study presents an alternative approach for identifying protein biomarkers to detect coaggregation abilities in uncharacterized strains.