促进健康的副杆菌,这是核心微生物组的一部分,最近受到了很多关注,显示其宿主的有益特性和作为新的生物治疗产品的潜力。然而,目前还没有研究研究使其维持在肠道微生物群中的分布式疟原虫的细胞表面分子和结构。此外,尽管双歧杆菌被强烈认为是一种肠道共生物种,对其宿主有好处,几部作品显示出有争议的结果,显示它是一种机会病原体。在这项研究中,我们报道了可能参与胶囊合成的基因簇,26个菌毛样和菌毛样细胞表面结构。并应用新的RfbA分型分类,以便更好地理解和表征与菌毛相关的有益/致病行为。两种不同类型的菌毛,在研究的26个基因组中鉴定出三种不同类型的菌毛和多达14个荚膜多糖基因座。此外,通过重排rfbA基因并在分类中增加第五组,将数据添加到rfbA型分类中改变了结局.总之,菌株在外部蛋白质结构方面的变异性可以解释先前观察到的品系间差异。但没有鉴定出与双歧杆菌有益或有害活性相关的特定结构。
The health-promoting Parabacteroides distasonis, which is part of the core microbiome, has recently received a lot of attention, showing beneficial properties for its host and potential as a new biotherapeutic product. However, no
study has yet investigated the cell surface molecules and structures of P. distasonis that allow its maintenance within the gut microbiota. Moreover, although P. distasonis is strongly recognized as an intestinal commensal species with benefits for its host, several works displayed controversial results, showing it as an opportunistic pathogen. In this
study, we reported gene clusters potentially involved in the synthesis of capsule, fimbriae-like and pili-like cell surface structures in 26 P. distasonis genomes and applied the new RfbA-typing classification in order to better understand and characterize the beneficial/pathogenic behavior related to P. distasonis strains. Two different types of fimbriae, three different types of pilus and up to fourteen capsular polysaccharide loci were identified over the 26 genomes studied. Moreover, the addition of data to the rfbA-type classification modified the outcome by rearranging rfbA genes and adding a fifth group to the classification. In conclusion, the strain variability in terms of external proteinaceous structure could explain the inter-strain differences previously observed of P. distasonis adhesion capacities and its potential pathogenicity, but no specific structure related to P. distasonis beneficial or detrimental activity was identified.