本研究旨在研究氨基官能化单宁酸模板介孔二氧化硅纳米颗粒(TA-MS-NH2NPs)对大鼠抗铁诱导的肝毒性的保护作用。为此,使用场发射扫描电子显微镜(FE-SEM)表征TA-MS-NH2NP,透射电子显微镜(TEM),动态光散射(DLS),和傅里叶变换红外光谱(FTIR)。此外,将50只Wistar大鼠随机分为对照组(1组)和4个实验组(2-5组)(n=10),每个分别接受100mg/kg口服生理盐水和FeSO4。然后,在两个间隔内测量暴露后的肝毒性和氧化应激标志物,即,在4和24小时后,其次是急性铁毒性的测量。此外,肝毒性标志物,包括丙氨酸转氨酶(ALT),天冬氨酸转氨酶(AST),碱性磷酸酶(ALP),和总抗氧化能力(TAC),通过铁还原抗氧化能力(FRAP)和2,2,1-二苯基-1-吡啶酰肼(DPPH)测定进行测量。此外,丙二醛(MDA),总硫醇基团,高级氧化蛋白产品(AOPP),和亚硝酸盐/硝酸盐(NOx)水平作为血清样品中的氧化应激标记物。结果表明,口服铁可显着提高肝酶并改变氧化应激标志物的水平。还发现,用TA-MS-NH2NP治疗有意义地保护免受肝毒性,ALT降低,AST,ALP,并通过降低MDA显著改善氧化应激标志物,AOPP,和NOx水平,增加TAC和硫醇基团含量,证明TA-MS-NH2NP可以通过其抗氧化特性保护大鼠免受铁诱导的急性肝毒性。
The present study sought to investigate the effects of amino-functionalized tannic acid-templated mesoporous silica nanoparticles (TA-MS-NH2 NPs) on giving rats protection against iron-induced liver toxicity. To this end, the TA-MS-NH2 NPs were characterized using field-emission scanning electron microscope (FE-SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), and Fourier-transform infrared spectroscopy (FTIR). Moreover, 50 Wistar rats were randomly divided into one control group (group 1) and four experimental groups (groups 2- 5) (n = 10), each of which received 100 mg/kg oral normal saline and FeSO4, respectively. Then, post-exposure hepatotoxicity and oxidative stress markers were measured in two intervals, i.e., after 4 and 24 h, followed by the measurement of the acute iron toxicity. Furthermore, hepatotoxicity markers, including the alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total antioxidant capacity (TAC), were measured via Ferric Reducing Antioxidant Power (FRAP) and 2,2,1-diphenyl-1-picrylhydrazyl (DPPH) assays. Also, malondialdehyde (MDA), total thiol groups, advanced oxidation protein products (AOPP), and nitrite/nitrate (NOx) levels were measured as oxidative stress markers in the serum samples. The results indicated that oral administration of iron significantly elevated the liver enzymes and altered the level of oxidative stress markers. It was also found that treatment with TA-MS-NH2 NPs meaningfully protected against hepatotoxicity, decreased ALT, AST, ALP, and significantly improved oxidative stress markers by decreasing MDA, AOPP, and NOx levels and increasing TAC and thiol group contents, proving that TA-MS-NH2 NPs could protect rats against iron-induced acute liver toxicity through their antioxidant features.