UNASSIGNED:细胞产品转换过程存在交叉污染的高风险。因此,在处理细胞产品时尽量减少交叉污染是至关重要的。在使用之后,生物安全柜的表面通常通过乙醇喷雾和手动擦拭方法进行消毒。然而,该方案的有效性和最佳消毒剂尚未评估。这里,我们评估了各种消毒剂和手动擦拭方法对细胞处理过程中细菌去除的影响。
UNASSIGNED:进行了硬质表面载体测试,以评估苯扎氯铵与腐蚀抑制剂(BKCI)的消毒剂功效,乙醇(ETH),过乙酸(PAA),并擦拭枯草芽孢杆菌内生孢子。蒸馏水(DW)用作对照。采用压力传感器来研究干燥和潮湿条件下的负载差异。由八名操作者使用湿时变黑的纸监测用于擦拭的预喷雾。化学性质,包括残留的漂浮蛋白,和机械性能,如粘度和摩擦系数,进行了检查。
未经批准:总共,对于BKCI和PAA,观察到枯草芽孢杆菌内生孢子的6-LogCFU减少了2.02±0.21-Log和3.00±0.46-Log,分别,治疗5分钟后。同时,在干燥条件下擦拭导致0.70±0.12-Log减少。在潮湿条件下,DW和BKC+I显示3.20±0.17-Log和3.92±0.46-Log减少,而ETH导致1.59±0.26-Log减少。对压力传感器的分析表明,力在干燥条件下没有传递。八位操作员对喷涂量的评估显示出喷涂区域的差异和偏差。虽然ETH在蛋白质漂浮和收集测定中的比例最低,它表现出最高的粘度。BKC+I在4.0-6.3mm/s下的摩擦系数最高;但是,在39.8-63.1mm/s下,BKC+I的摩擦系数下降,与ETH的摩擦系数相似。
UNASSIGNED:DW和BKC+I对于诱导细菌丰度的3-Log减少是有效的。此外,最佳湿润条件和消毒剂的组合对于在含有高蛋白人血清和组织的特定环境中有效擦拭至关重要。鉴于在细胞产品中加工的一些原材料含有较高的蛋白质水平,我们的研究结果表明,在清洁和消毒方面,生物安全柜的完全转换是必要的。
UNASSIGNED: The process of cell product changeover poses a high risk of cross-contamination. Hence, it is essential to minimize cross-contamination while processing cell products. Following its use, the surface of a biosafety cabinet is commonly disinfected by ethanol spray and manual wiping methods. However, the effectiveness of this protocol and the optimal disinfectant have not yet been evaluated. Here, we assessed the effect of various disinfectants and manual wiping methods on bacterial removal during cell processing.
UNASSIGNED: The hard surface carrier test was performed to evaluate the disinfectant efficacy of benzalkonium chloride with a corrosion inhibitor (BKC + I), ethanol (ETH), peracetic acid (PAA), and wiping against Bacillus subtilis endospores. Distilled water (DW) was used as the control. A pressure sensor was employed to investigate the differences in loading under dry and wet conditions. The pre-spray for wiping was monitored by eight operators using a paper that turns black when wet. Chemical properties, including residual floating proteins, and mechanical properties, such as viscosity and coefficient of friction, were examined.
UNASSIGNED: In total, 2.02 ± 0.21-Log and 3.00 ± 0.46-Log reductions from 6-Log CFU of B. subtilis endospores were observed for BKC + I and PAA, respectively, following treatment for 5 min. Meanwhile, wiping resulted in a 0.70 ± 0.12-Log reduction under dry conditions. Under wet conditions, DW and BKC + I showed 3.20 ± 0.17-Log and 3.92 ± 0.46-Log reductions, whereas ETH caused a 1.59 ± 0.26-Log reduction. Analysis of the pressure sensor suggested that the force was not transmitted under dry conditions. Evaluation of the amount of spray by eight operators showed differences and bias in the spraying area. While ETH had the lowest ratio in the protein floating and collection assays, it exhibited the highest viscosity. BKC + I had the highest friction coefficient under 4.0-6.3 mm/s; however, that of BKC + I decreased and became similar to the friction coefficient of ETH under 39.8-63.1 mm/s.
UNASSIGNED: DW and BKC + I are effective for inducing a 3-Log reduction in bacterial abundance. Moreover, the combination of optimal wet conditions and disinfectants is essential for effective wiping in specific environments containing high-protein human sera and tissues. Given that some raw materials processed in cell products contain high protein levels, our findings suggest that a complete changeover of biosafety cabinets is necessary in terms of both cleaning and disinfection.