关键词： ACSA-2, astrocyte cell surface antigen-2 Astrocytes BrdU, 5-bromo-2′-deoxyuridine Cerebral ischemia DEG, differentially expressed gene EDTA, ethylenediaminetetraacetic acid FBS, fetal bovine serum GFAP, glial fibrillary acidic protein GO, gene ontology GST-π, glutathione S-transferase-π Gstp1, glutathione S-transferase, pi 1 Gstp2, glutathione S-transferase, pi 2 Iba1, ionized calcium-binding adapter molecule 1 Ig, immunoglobulin Lcn2, lipocalin 2 MCAO, middle cerebral artery occlusion PBS, phosphate-buffered saline PFA, 4% paraformaldehyde POD, post-operative day Proliferation TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick 3’-end labeling Transcriptome Vegfa, vascular endothelial growth factor A Voluntary running exercise Vtn, vitronectin qPCR, quantitative polymerase chain reaction

来  源：   DOI：10.1016/j.ibneur.2023.02.004   PDF(Pubmed)

Abstract：
Rehabilitative exercise following a brain stroke has beneficial effects on the morphological plasticity of neurons. Particularly, voluntary running exercise after focal cerebral ischemia promotes functional recovery and ameliorates ischemia-induced dendritic spine loss in the peri-infarct motor cortex layer 5. Moreover, neuronal morphology is affected by changes in the perineuronal environment. Glial cells, whose phenotypes may be altered by exercise, are known to play a pivotal role in the formation of this perineuronal environment. Herein, we investigated the effects of voluntary running exercise on glial cells after middle cerebral artery occlusion. Voluntary running exercise increased the population of glial fibrillary acidic protein-positive astrocytes born between post-operative days (POD) 0 and 3 on POD15 in the peri-infarct cortex. After exercise, transcriptomic analysis of post-ischemic astrocytes revealed 10 upregulated and 70 downregulated genes. Furthermore, gene ontology analysis showed that the 70 downregulated genes were significantly associated with neuronal morphology. In addition, exercise reduced the number of astrocytes expressing lipocalin 2, a regulator of dendritic spine density, on POD15. Our results suggest that exercise modifies the composition of astrocytic population and their phenotype.
摘要：
中风后的康复运动对神经元的形态可塑性具有有益的影响。特别是,局灶性脑缺血后的自愿跑步运动可促进功能恢复，并改善梗死周围运动皮质层5的缺血诱导的树突棘丢失。此外，神经元形态受神经周环境变化的影响。胶质细胞,其表型可能因运动而改变，众所周知，在这种神经周环境的形成中起着关键作用。在这里，我们研究了自主跑步运动对大脑中动脉闭塞后神经胶质细胞的影响。自愿跑步运动增加了梗死周围皮质POD15术后0和3天（POD）之间出生的胶质纤维酸性蛋白阳性星形胶质细胞的数量。锻炼后,缺血后星形胶质细胞的转录组学分析显示10个上调和70个下调的基因。此外，基因本体论分析表明，70个下调基因与神经元形态显著相关。此外,运动减少了表达脂质运载蛋白2的星形胶质细胞的数量，POD15我们的结果表明，运动改变了星形细胞种群的组成及其表型。
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