■外泌体样纳米颗粒(ELN)介导种间细胞间通讯并调节基因表达。
■在这项研究中,我们从苍术干根茎中分离和纯化了ELN(Thunb。)DC。[菊科](ALR-ELNs),一种传统的天然药物,并研究了它们作为神经炎症治疗剂的潜力。
■使用差速离心分离和纯化ALR-ELN样品,并通过透射电子显微镜和RNA测序分析了它们的物理特征和microRNA含量,分别。BV-2小鼠小胶质细胞和原代小鼠小胶质细胞体外培养,使用荧光显微镜研究了它们摄取ALR-ELN的能力。通过mRNA和蛋白质表达分析评估ALR-ELN调节这些细胞对脂多糖(LPS)暴露的抗炎反应的能力。
■总的来说,发现BV-2细胞内化ALR-ELN,其中包含三个microRNA(ath-miR166f,ath-miR162a-5p,和ath-miR162b-5p)可能具有抗炎活性。用ALR-ELN预处理BV-2细胞通过显着降低一氧化氮水平来预防LPS刺激的促炎作用,白细胞介素-1β,白细胞介素-6和肿瘤坏死因子-α。值得注意的是,Il1b的mRNA水平,Il6iNos,BV-2细胞中的ccl2和cxcl10,随着LPS的暴露而增加,ALR-ELN治疗后显著降低。此外,ALR-ELN处理后,血红素加氧酶1、Irf7、ccl12和Irg1的mRNA水平也显著增加。此外,用ALR-ELN预处理原代小鼠小胶质细胞通过显着降低一氧化氮水平来预防LPS刺激的促炎作用。
■我们的发现表明ALR-ELN对小鼠小胶质细胞表现出抗炎作用。进一步的验证可能证明ALR-ELN是一种有前途的神经炎性治疗剂。
UNASSIGNED: Exosome-like nanoparticles (ELNs) mediate interspecies intercellular communications and modulate gene expression.
UNASSIGNED: In this study, we isolated and purified ELNs from the dried rhizome of Atractylodes lancea (Thunb.) DC. [Asteraceae] (ALR-ELNs), a traditional natural medicine, and investigated their potential as neuroinflammatory therapeutic agents.
UNASSIGNED: ALR-ELN samples were isolated and purified using differential centrifugation, and their physical features and microRNA contents were analyzed through transmission electron microscopy and RNA sequencing, respectively. BV-2 microglial murine cells and primary mouse microglial cells were cultured in vitro, and their ability to uptake ALR-ELNs was explored using fluorescence microscopy. The capacity of ALR-ELNs to modulate the anti-inflammatory responses of these cells to lipopolysaccharide (LPS) exposure was assessed through mRNA and protein expression analyses.
UNASSIGNED: Overall, BV-2 cells were found to internalize ALR-ELNs, which comprised three microRNAs (ath-miR166f, ath-miR162a-5p, and ath-miR162b-5p) that could have anti-inflammatory activity. Pretreatment of BV-2 cells with ALR-ELN prevented the pro-inflammatory effects of LPS stimulation by significantly reducing the levels of nitric oxide, interleukin-1β, interleukin-6, and tumor necrosis factor-α. Notably, the mRNA levels of Il1b, Il6, iNos, ccl2, and cxcl10 in BV-2 cells, which increased upon LPS exposure, were significantly reduced following ALR-ELN treatment. Moreover, the mRNA levels of heme oxygenase 1, Irf7, ccl12, and Irg1 also increased significantly following ALR-ELN treatment. In addition, pretreatment of primary mouse microglial cells with ALR-ELN prevented the pro-inflammatory effects of LPS stimulation by significantly reducing the levels of nitric oxide.
UNASSIGNED: Our findings indicate that ALR-ELNs exhibit anti-inflammatory effects on murine microglial cells. Further validation may prove ALR-ELNs as a promising neuroinflammatory therapeutic agent.