Eustrongylides是一种鱼类传播的人畜共患寄生虫,已知会感染各种鱼类,包括北派克(EsoxLucius)。这种线虫,属于科章鱼科,具有涉及多个主机的复杂生命周期。本研究旨在调查北梭鱼中Eustrongylides线虫的发生(E。Lucius)从Mijran大坝(拉姆萨尔,伊朗)。在2023年6月至10月之间,对拉姆萨尔的Mijran大坝的NorthernPike进行了调查,伊朗,以下报道的红色寄生虫在他们的肌肉组织。德黑兰大学对60条鱼进行了检查,揭示肌肉中的活寄生虫,然后对其进行显微镜分析并保存以进行多学科研究。85%(51/60)的鱼类标本的骨骼肌组织被肉眼可见的幼虫感染,这些幼虫在显微镜下被鉴定为Eustrongylidesspp。在组织病理学检查中,病变由包裹性寄生性肉芽肿性肌炎组成。微观上,囊性寄生性肉芽肿压迫了邻近的肌纤维,导致它们的萎缩和Zenker的坏死.此外,上皮样巨噬细胞,巨细胞和单核炎症细胞存在于幼虫周围和肌纤维之间。最后,通过检查ITS基因区域进行分子分析,揭示它们属于E.excisus物种。伊朗北部的Eustrongylidiasis需要对生物学进行进一步研究,流行病学,和控制Eustrongylides线虫,专注于各种主机。这项研究是第一个全面描述拉姆萨尔北部梭鱼的E.uctrus,伊朗,引起人们对可能的人畜共患传播的担忧。
Eustrongylides excisus is a fish-borne zoonotic parasite known to infect various fish species, including Northern pike (Esox Lucius). This nematode, belonging to the family Dioctophymatidae, has a complex life cycle involving multiple hosts. This study aimed to investigate the occurrence of Eustrongylides nematodes in Northern pike (E. Lucius) collected from Mijran Dam (Ramsar, Iran). Between June and October 2023, an investigation was conducted on Northern pike from Mijran Dam in Ramsar, Iran, following reports of reddish parasites in their muscle tissues. Sixty fish were examined at the University of Tehran, revealing live parasites in the muscles, which were then analyzed microscopically and preserved for a multidisciplinary study. The skeletal muscle tissues of 85% (51/60) of fish specimens were infected by grossly visible larvae which were microscopically identified as Eustrongylides spp. In histopathological examination, the lesion was composed of encapsulated parasitic granulomatous myositis. Microscopically, the cystic parasitic granulomas compressed the adjacent muscle fibers, leading to their atrophy and Zenker\'s necrosis. Moreover, epithelioid macrophages, giant cells and mononuclear inflammatory cells were present around the larvae and between the muscle fibers. Finally, a molecular analysis by examining the ITS gene region, revealed that they belong to the species E. excisus. Eustrongylidiasis in northern Iran necessitates further research into the biology, epidemiology, and control of Eustrongylides nematodes, focusing on various hosts. This study is the first to comprehensively characterize E. excisus in Northern pike in Ramsar, Iran, raising concerns about possible zoonotic transmission.