背景技术草药已经在医学实践中使用了几个世纪,并且继续在现代补充和替代医学中发挥重要作用。这些草药中的植物化学物质具有很强的抗氧化和抗炎特性,这有利于解决口腔健康问题,如牙菌斑,牙龈炎,和口腔微生物感染。随着研究的进展,挑战仍然是将这些天然化合物转化为安全的,有效,以及各种疾病的可获得治疗。目的本研究的目的是配制苦参和紫锥菊凝胶,以及抗菌评价,抗炎,自由基清除活性,和细胞毒性潜力。材料和方法使用向其中加入100ml蒸馏水的印度苦素和紫锥菊的浓缩粉末混合物(各5克)制备印度苦素和紫锥菊凝胶。将混合物在60℃下煮沸30分钟。将10ml浓缩物与20ml卡波姆和羧甲基纤维素(CMC)混合物混合并充分混合。这就产生了印度和紫锥菊凝胶。然后,抗菌剂,抗炎,细胞毒性潜能,并对凝胶的自由基清除活性进行了评价。所得数据借助配对t检验进行统计分析,其中p值小于0.05被认为具有统计学意义。结果抗菌试验表明,在100微克浓度下,印度和紫锥菊凝胶对金黄色葡萄球菌的抑制作用更大(3.15±0.26),变形链球菌(2.48±0.45),粪肠球菌(2.89±0.15),白色念珠菌(4.28±0.87)。细胞毒性试验显示,即使在80微克浓度的提取物,超过70%的无节幼体是至关重要的,这表明该凝胶没有细胞毒性。与双氯芬酸钠(73.16±1.80)相比,凝胶的抗炎活性最高(78.39±1.82)为50微克。自由基清除活性表明,印度和紫锥菊提取物的2,2-二苯基-1-苦基肼基(DPPH)吸光度最高,为50微克。结论与标准药物相比,苦艾和紫锥菊提取物凝胶具有较高的抗菌和抗炎活性。如阿莫西林和双氯芬酸钠。凝胶的抗氧化活性等于丁基化羟基甲苯(BHT),并且该凝胶即使在其较高浓度下也具有低细胞毒性潜力。因此,该凝胶可用作天然药物,副作用最小,使其成为化学试剂的有价值的替代品。
Background Herbs have been used in medical practice for centuries and continue to play a significant role in modern complementary and alternative medicine. Phytochemicals in these herbs possess strong antioxidant and anti-inflammatory properties, which are beneficial in targeting oral health issues, such as dental plaque, gingivitis, and oral microbial infections. As research progresses, the challenge remains to translate these natural compounds into safe, effective, and accessible treatments for a wide range of diseases. Aim The aim of this research was to formulate the neem and
echinacea gel along with the evaluation of antimicrobial, anti-inflammatory, free-radical scavenging activity, and cytotoxic potential. Materials and methods The neem and
echinacea gel was prepared using a concentrated powdered mixture of neem and
echinacea (5 grams each) to which 100 ml of distilled water was added, and the mixture was boiled for 30 minutes at 60°C. The 10 ml concentrate was mixed with 20 ml of a carbopol and carboxymethyl cellulose (CMC) mixture and mixed thoroughly, which resulted in neem and
echinacea gel. Then, the antimicrobial, anti-inflammatory, cytotoxic potential, and free-radical scavenging activity of the gel were evaluated. The data obtained were statistically analyzed with the help of a paired t-test, where a p-value of less than 0.05 was considered statistically significant. Results The antimicrobial assay showed that neem and
echinacea gel at the concentration of 100 micrograms showed a greater zone of inhibition against Staphylococcus aureus (3.15 ± 0.26), Streptococcus mutans (2.48 ± 0.45), Enterococcus faecalis (2.89 ± 0.15), and Candida albicans (4.28 ± 0.87). The cytotoxic test revealed that even at an 80 µg concentration of the extract, more than 70% of the nauplii were vital, which indicated that the gel was not cytotoxic. The highest anti-inflammatory activity (78.39 ± 1.82) of the gel was seen at 50 micrograms when compared with diclofenac sodium (73.16 ± 1.80). The free radical scavenging activity showed that the 2,2-diphenyl-1-picrylhydrazyl (DPPH) absorbance of the neem and echinacea extract was highest at 50 micrograms. Conclusion The combination of neem and echinacea extract-based gel possessed high antimicrobial and anti-inflammatory activity when compared with standard drugs, such as amoxicillin and diclofenac sodium. The antioxidant activity of the gel was equal to butylated hydroxytoluene (BHT), and also the gel has a low cytotoxic potential even at its higher concentrations. Hence, the gel can be used as a natural remedy with minimal side effects, making it a valuable alternative to chemical agents.