■两种新鉴定的蛋白质,EspB和EspC参与结核分枝杆菌的发病机制。本研究的目的是评估重组EspC的免疫原性,EspB,和小鼠中的EspC/EspB融合蛋白。
■用重组EspC皮下免疫BALB/c小鼠,EspB,和融合的EspC/EspB蛋白,与Quil-A一起作为佐剂。通过定量IFN-γ评估细胞和体液免疫应答,IL-4,IgG,针对抗原的IgG1和IgG2a抗体。
■结果表明,用重组EspC免疫小鼠,EspB,和EspC/EspB蛋白不产生IL-4,而IFN-γ响应于所有三种蛋白而分泌。EspC/EspB组响应于所有三种重组蛋白的刺激产生显著量的IFN-γ(P<0.001)。在用EspC免疫的小鼠中,检测到高水平的IFN-γ响应EspC/EspB,和EspC(P<0.0001);而用EspB免疫的小鼠响应EspC/EspB产生较低水平的IFN-γ,和EspB(P<0.05)。重组EspC免疫小鼠,EspB,和EspC/EspB蛋白表现出显著高水平的IgG和IgG2a/IgG1比率(P<0.001)。此外,在用EspC/EspB融合蛋白免疫的小鼠血清中检测到高水平的IgG和IgG2a。
■这三种重组蛋白均能诱导小鼠Th1型免疫应答,抗EspB和EspC;由于来自EspC和EspB蛋白两者的表位的存在以及针对两者的免疫应答的产生,EspC/EspB蛋白是更期望的。
UNASSIGNED: Two newly identified proteins, EspB and EspC are involved in the pathogenesis of Mycobacterium tuberculosis. The objective of the present study was to evaluate the immunogenicity of recombinant EspC, EspB, and EspC/
EspB fusion proteins in mice.
UNASSIGNED: BALB/c mice were immunized subcutaneously with recombinant EspC, EspB, and fusion EspC/
EspB proteins, three times with along with Quil-A as an adjuvant. The cellular and humoral immune responses were evaluated by quantifying IFN-γ, IL-4, IgG, IgG1, and IgG2a antibodies against the antigens.
UNASSIGNED: The results showed that the mice immunized with recombinant EspC, EspB, and EspC/EspB proteins did not produce IL-4, whereas IFN-γ was secreted in response to all three proteins. EspC/EspB group produced significant amounts of IFN-γ in response to stimulation with all the three recombinant proteins (P<0.001). In mice immunized with EspC, high levels of IFN-γ were detected in response to EspC/EspB, and EspC (P<0.0001); while mice immunized with
EspB produced lower levels of IFN-γ in response to EspC/
EspB, and
EspB (P<0.05).Mice immunized with recombinant EspC,
EspB, and EspC/
EspB proteins exhibited significantly high levels of IgG and IgG2a/IgG1 ratio (P< 0.001). Moreover, high levels of IgG and IgG2a were detected in the sera of mice immunized with EspC/EspB fusion protein.
UNASSIGNED: All the three recombinant proteins induced Th1-type immune responses in mice against EspB and EspC; however, EspC/EspB protein is more desirable due to the presence of epitopes from both EspC and
EspB proteins and the production of immune responses against both.