目的:本研究旨在探讨EB病毒(EBV)阳性炎性滤泡树突状细胞肉瘤(IFDCS;EBV+IFDCS)的临床病理特征。
方法:该病例涉及一名32岁女性,她接受了脾结节的手术切除。组织学检查和免疫组织化学使用分化簇(CD)标记进行,进行原位杂交以检测EBV编码的RNA(EBER)。
结果:显微镜分析显示肿瘤细胞具有不同的形态,包括圆形,卵形,或者纺锤状的形状,分散在突出的淋巴浆细胞浸润中。肿瘤细胞表现出核异型,有一些类似里德-斯特恩伯格的细胞。免疫组织化学显示滤泡树突状细胞标志物的局灶性阳性,如CD21、CD23和CD35,以及其他标志物的局灶性阴性,包括CD3、CD34、CD20、CD79a、髓过氧化物酶和HMB45。此外,EBER染色显示强阳性结果.随访13个月,患者未见局部复发或转移。
结论:全面了解EBV+IFDCS,包括其临床病理特征和免疫组织化学特征,对于这种罕见肿瘤的准确诊断和鉴别诊断至关重要。
OBJECTIVE: The present study aims to explore the clinicopathological characteristics of Epstein-Barr virus (EBV)-positive inflammatory follicular dendritic cell sarcoma (IFDCS; EBV+ IFDCS).
METHODS: The case involved a 32-year-old woman who underwent surgical resection of a splenic nodule. Histological examination and immunohistochemistry were performed using cluster of differentiation (CD) markers, and in-situ hybridization was conducted to detect EBV-encoded RNA (EBER).
RESULTS: A microscopic analysis revealed neoplastic cells with various morphologies, including round, ovoid, or spindled shapes, dispersed within a prominent lymphoplasmacytic infiltrate. The tumor cells exhibited nuclear atypia, with some resembling Reed-Sternberg cells. The immunohistochemistry demonstrated focal positivity for follicular dendritic cell markers, such as CD21, CD23 and CD35, and focal negativity for other markers, including CD3, CD34, CD20, CD79a, myeloperoxidase and HMB45. Additionally, the EBER staining showed strongly positive results. The patient showed no local recurrence or metastasis during the 13-month follow-up.
CONCLUSIONS: A comprehensive understanding of EBV+IFDCS, including its clinicopathological features and immunohistochemical characteristics, is crucial for accurate diagnosis and differential diagnosis of this rare tumor.