将双足体植物暴露于th(Tl)胁迫(10、50和100µM)7天。Tl毒性改变了其他营养素的吸收和积累。在根部和叶子中,K下降了,Mg,和Fe含量,但是Ca的增加,Mn,和Zn。叶绿素减少,光合效率也是如此,而类胡萝卜素增加。根部的氧化应激反映在脂质过氧化作用增加。有更多的超氧化物(O2。-),过氧化氢(H2O2),和一氧化氮(NO)在根部比在叶子,随着两个器官对Tl毒性的反应增加,除了O2.-在根部生产,波动。硫化氢(H2S)产量增加,尤其是在叶子上。超氧化物歧化酶(SOD),抗坏血酸过氧化物酶(APX),脱氢抗坏血酸还原酶(DHAR),单脱氢抗坏血酸还原酶(MDHAR),谷胱甘肽还原酶(GR)活性增加,除了根中的APX和MDHAR和叶子中的GR。抗坏血酸-谷胱甘肽循环的成分受到影响。因此,抗坏血酸(AsA)增加,而脱氢抗坏血酸(DHA),还原型谷胱甘肽(GSH),氧化型谷胱甘肽(GSSG)减少,除了在100µMTl的根部,显示GSH增加。这些Tl毒性诱导的改变改变了AsA/DHA和GSH/GSSG氧化还原状态。NO和H2S的相互作用可以通过激活抗氧化剂系统而起作用。Tl的作用可能与其与-SH基团结合的强亲和力有关,从而改变蛋白质的功能和细胞的氧化还原状态。
Dittrichia plants were exposed to thallium (Tl) stress (10, 50, and 100 µM) for 7 days. The Tl toxicity altered the absorption and accumulation of other nutrients. In both the roots and the leaves, there was a decline in K, Mg, and Fe content, but an increase in Ca, Mn, and Zn. Chlorophylls decreased, as did the photosynthetic efficiency, while carotenoids increased. Oxidative stress in the roots was reflected in increased lipid peroxidation. There was more production of superoxide (O2.-), hydrogen peroxide (H2O2), and nitric oxide (NO) in the roots than in the leaves, with increases in both organs in response to Tl toxicity, except for O2.- production in the roots, which fluctuated. There was increased hydrogen sulfide (H2S) production, especially in the leaves. Superoxide dismutase (SOD), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR), and glutathione reductase (GR) showed increased activities, except for APX and MDHAR in the roots and GR in the leaves. The components of the ascorbate-glutathione cycle were affected. Thus, ascorbate (AsA) increased, while dehydroascorbate (DHA), reduced glutathione (GSH), and oxidized glutathione (GSSG) decreased, except for in the roots at 100 µM Tl, which showed increased GSH. These Tl toxicity-induced alterations modify the AsA/DHA and GSH/GSSG redox status. The NO and H2S interaction may act by activating the antioxidant system. The effects of Tl could be related to its strong affinity for binding with -SH groups, thus altering the functionality of proteins and the cellular redox state.