■万寿菊,俗称万寿菊,具有多种药理作用。它被用作膳食补充剂,尤其是眼睛的后段。然而,T.eriptalinn对眼部疾病的影响尚不清楚。目的探讨小鼠模型口服直角草花乙醇提取物(TE)对干眼综合征(DED)的影响。
■使24只小鼠经受干燥应激(DS)以诱导DED,并且每天4次皮下注射氢溴酸东莨菪碱,持续21天。在DS条件下再施用TE和环孢菌素A(CsA)14天。小鼠随机分为四组:对照组,TE200、TE400和CsA。在基线测量泪液产生和角膜荧光素染色的变化,DS7天后,治疗后7天和14天。
■DS显着降低泪液产生并增加角膜荧光素评分;在TE400(口服400mgTE/kg体重)组中,参数显着逆转。TE显着改善了DS引起的变化,包括角膜上皮脱离和泪腺炎症。TE400补充剂的抗炎作用与CsA相似。
■我们的发现表明,口服TE可以通过稳定泪膜和抑制炎症来预防DS诱导的DED。本研究为进一步研究TE预防DED的临床应用提供了实验基础。
UNASSIGNED: Tagetes erecta Linn, popularly known as Marigold, has various pharmacological effects. It is used as a dietary supplement, especially for the posterior segment of the eye. However, the effect of T. erecta Linn on ocular disorders is still unknown. The purpose of this study was to investigate the effect of oral administration of ethanol extract of T. erecta Linn flower (TE) for dry eye syndrome (DED) in a murine model.
UNASSIGNED: Twenty-four mice were subjected to desiccation stress (DS) to induce DED and subcutaneous injection of scopolamine hydrobromide was administered 4 times a day for 21 days. TE and cyclosporine A (CsA) were administered for an additional 14 days under DS conditions. Mice were randomly divided into four groups: control, TE200, TE400, and CsA. Changes in tear production and corneal fluorescein staining were measured at baseline, after 7 days of DS, and after treatment for 7 and 14 days.
UNASSIGNED: DS significantly decreased tear production and increased corneal fluorescein score; the parameters were significantly reversed in the TE400 (oral administration of 400 mg TE/kg body weight) group. TE markedly improved DS-induced changes including corneal epithelial detachment and lacrimal gland inflammation. The anti-inflammatory effect of TE 400 supplementation was similar to that of CsA.
UNASSIGNED: Our findings suggest that oral administration of TE may protect against DS-induced DED via stabilization of the tear film and suppression of inflammation. This study provides an experimental basis for further studies on the potential clinical use of TE in preventing DED.