Abstract：
CRF-like diuretic hormone receptor (CRF/DHR), also known as DH44R in insects, are G-protein coupled receptors (GPCRs) that play a role in regulating osmotic balance in various insect species. These receptors have the potential to be targeted for the development of insecticides. However, our understanding of the role of DHR genes in aphids, including Rhopalosiphum padi, a major wheat pest, is currently limited. In this study, we isolated and characterized two R. padi DHRs (RpDHR1 and RpDHR2). The expression levels of RpDHR1 increased after starvation and were restored after re-feeding. The expression levels of RpDHR1 gene decreased significantly 24 h after injection of dsRNA targeting the gene. Knockdown of RpDHR1 increased aphid mortality under starvation conditions (24, 36, 48 and 60 h). Under starvation and desiccation condition, the aphid mortality decreased after knockdown of RpDHR1. This is the first study to report the role of DHR genes in the starvation and desiccation response of aphids. The results suggest that RpDHR1 is involved in the resistance of R. padi to starvation and dehydration, making it a potential target for insecticide development. Novel insecticides could be created by utilizing DHR agonists to disrupt the physiological processes of insect pests.
摘要：
CRF样利尿激素受体(CRF/DHR),在昆虫中也被称为DH44R，G蛋白偶联受体（GPCRs）在调节各种昆虫物种的渗透平衡中起作用。这些受体有可能成为开发杀虫剂的目标。然而,我们对DHR基因在蚜虫中的作用的理解，包括Rhapalosiphumpadi,一种主要的小麦害虫，目前有限。在这项研究中,我们分离并表征了两个R.padiDHR(RpDHR1和RpDHR2)。RpDHR1的表达水平在饥饿后增加，并在重新喂食后恢复。注射靶向该基因的dsRNA后24小时，RpDHR1基因的表达水平显着降低。在饥饿条件下（24、36、48和60小时），敲除RpDHR1会增加蚜虫的死亡率。在饥饿和干燥条件下，敲除RpDHR1后，蚜虫死亡率降低。这是首次报道DHR基因在蚜虫饥饿和干燥反应中的作用的研究。结果表明，RpDHR1参与了R.padi对饥饿和脱水的抗性，使其成为杀虫剂开发的潜在目标。可以通过利用DHR激动剂破坏昆虫害虫的生理过程来产生新型杀虫剂。
