此处描述的数据提供了鼠原始造血干细胞和祖细胞(LSK)和B细胞群的全基因组表达谱,通过高通量测序获得。细胞来源于野生型小鼠和缺乏泛素特异性蛋白酶3(USP3;Usp3Δ/Δ)的小鼠。泛素对组蛋白的修饰在细胞对DNA损伤(DDR)的反应中起着至关重要的作用(Jackson和Durocher,2013)[1]。USP3是一种组蛋白H2A去泛素化酶(DUB),可调节泛素依赖性DDR以响应DNA双链断裂(Nicassio等人。,2007;Doil等人。,2008)[2],[3].在小鼠中删除USP3会增加自发性肿瘤的发生率并影响造血[4]。特别是,Usp3敲除小鼠在衰老过程中显示B和T细胞的进行性丧失和造血干细胞(HSC)的功能潜能降低。USP3缺陷细胞,包括HSC,显示增强的组蛋白泛素化,积累自发的DNA损伤,对电离辐射过敏(Lancini等人。,2014年)[4]。为了解决USP3丢失是否导致与HSC稳态和/或B细胞发育相关的特定分子途径的失调,我们采用了RNA测序技术,研究了野生型和Usp3Δ/ΔLSK之间的转录差异,原始B细胞或体外激活的B细胞。数据与研究文章“USP3对泛素介导的DNA损伤反应的严格调节保留了造血干细胞的功能完整性”有关(Lancini等人。,2014年)[4]。RNA测序和分析数据集已保存在NCBI的基因表达Omnibus中(Edgar等人。,2002)[5],可通过GEO系列登录号GSE58495(http://www。ncbi.nlm.nih.gov/geo/query/acc。cgi?acc=GSE58495)。有了这篇文章,我们通过定量实时PCR和比较分析验证了RNA-seq数据集.
The data described here provide genome-wide expression profiles of murine primitive hematopoietic stem and progenitor cells (LSK) and of B cell populations, obtained by high throughput sequencing. Cells are derived from wild-type mice and from mice deficient for the ubiquitin-specific protease 3 (USP3; Usp3Δ/Δ). Modification of histone proteins by ubiquitin plays a crucial role in the cellular response to DNA damage (DDR) (Jackson and Durocher, 2013) [1]. USP3 is a histone H2A deubiquitinating enzyme (DUB) that regulates ubiquitin-dependent DDR in response to DNA double-strand breaks (Nicassio et al., 2007; Doil et al., 2008) [2], [3]. Deletion of USP3 in mice increases the incidence of spontaneous tumors and affects hematopoiesis [4]. In particular, Usp3-knockout mice show progressive loss of B and T cells and decreased functional potential of hematopoietic stem cells (HSCs) during aging. USP3-deficient cells, including HSCs, display enhanced histone ubiquitination, accumulate spontaneous DNA damage and are hypersensitive to ionizing radiation (Lancini et al., 2014) [4]. To address whether USP3 loss leads to deregulation of specific molecular pathways relevant to HSC homeostasis and/or B cell development, we have employed the RNA-sequencing technology and investigated transcriptional differences between wild-type and Usp3Δ/Δ LSK, naïve B cells or in vitro activated B cells. The data relate to the research article \"Tight regulation of ubiquitin-mediated DNA damage response by USP3 preserves the functional integrity of hematopoietic stem cells\" (Lancini et al., 2014) [4]. The RNA-sequencing and analysis data sets have been deposited in NCBI׳s Gene Expression Omnibus (Edgar et al., 2002) [5] and are accessible through GEO Series accession number GSE58495 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE58495). With this article, we present validation of the RNA-seq data set through quantitative real-time PCR and comparative analysis.