■本研究旨在探讨多巴胺D2样受体(DRD2)基因-141C和C957T多态性在中国汉族血脂异常人群中的基因型和等位基因分布。以及它们与血脂水平的关系。
■招募150名血脂异常患者和150名健康人作为病例和对照组,分别。血清总胆固醇(TC),甘油三酯(TG),高密度脂蛋白胆固醇(HDL-C),检测低密度脂蛋白胆固醇水平。通过聚合酶链反应扩增DRD2多态性的靶序列,并通过Sanger测序进行基因分型。
■在DRD2基因C957T(rs6277)中,CC的三种基因型,CT,TT检测频率为92.67%,6.67%,在血脂异常病例中为0.67%,和83.33%,14.67%,对照组为2.00%,分别。病例组的CT基因型和T等位基因频率明显低于对照组。调整其他临床指标后,C957T多态性的CT基因型(风险比=0.401,95%置信区间=0.181-0.890,p<0.05)仍与血脂异常风险显著降低相关.CTC957T基因型携带者血清TC值最低,TG,LDL,和血清HDL-C的最高值
■DRD2基因C957T多态性是与血脂异常易感性相关的独立影响因素,CT基因型与血脂异常易感性降低相关。
UNASSIGNED: The study aimed to explore the genotype and allele distributions of dopamine D2-like receptor (
DRD2) gene -141C and C957T polymorphisms in the Chinese Han population with dyslipidemia, as well as their association with serum lipid levels.
UNASSIGNED: One hundred fifty patients with dyslipidemia and 150 healthy people were recruited as the case and the control groups, respectively. Serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol levels were detected. The target sequence of
DRD2 polymorphisms was amplified by polymerase chain reaction and genotyped via Sanger sequencing.
UNASSIGNED: In
DRD2 gene C957T (rs6277), three genotypes of CC, CT, and TT were detected with the frequencies of 92.67%, 6.67%, 0.67% in dyslipidemia cases, and 83.33%, 14.67%, 2.00% in the controls, respectively. The CT genotype and T allele frequencies were significantly low in the case group relative to the control group. After adjusting to other clinical indicators, the CT genotype of C957T polymorphism (hazard ratio = 0.401, 95% confidence interval = 0.181-0.890, p < 0.05) was still related to a significantly reduced risk of dyslipidemia. The C957T CT genotype carriers had the lowest values of serum TC, TG, LDL, and the highest values of serum HDL-C.
UNASSIGNED: DRD2 gene C957T polymorphism was an independent influencing factor associated with the susceptibility to dyslipidemia, and the CT genotype was associated with decreased odds of susceptibility to dyslipidemia.