■ErianthiopicumaethiopicumWiensandPolhill(Loranthaceae)是原产于东北非洲和埃塞俄比亚的寄生植物。在埃塞俄比亚,传统上用于治疗乳房肿胀,乳腺炎,早晨的疾病和呕吐。
■这项研究旨在筛选主要的植物化学成分;确定酚类物质的总量,黄酮类化合物,和单宁;并评估抗微生物剂(抗大肠杆菌,葡萄球菌,念珠菌和白隐球菌)和抗氧化(抗DPPH自由基和铁离子)活性。
■使用正己烷浸渍粉末的aethiopicum叶,氯仿,乙酸乙酯,乙醇,和甲醇。对所有粗提物进行定性筛选以进行植物化学鉴定。总酚类物质,类黄酮,和浓缩的氯仿中的单宁含量,乙醇,采用紫外-可见分光光度法测定甲醇提取物的含量。正己烷,氯仿,使用琼脂圆盘扩散和肉汤微稀释技术,评估了甲醇提取物对上述微生物的抗菌活性。氯仿,乙醇,还通过DPPH和铁离子还原抗氧化能力(FRAP)测定评估了甲醇提取物的抗氧化活性。
■甲醇(17.56±16%)和乙醇(16.45±19%)显示出更好的提取效率。黄酮类化合物,多酚,单宁,萜类化合物,皂苷,在所有提取物中检测到甾醇。酚类物质总含量最高(22.63±0.69mgGAE/gDCE),类黄酮(5.38±0.52mgCE/gDCE)和单宁(39.18±38mgCE/gDCE),每克干粗提物含没食子酸和儿茶素毫克数,记录在甲醇提取物中。甲醇提取物还表现出最好的抗DPPH强度(IC50,4.31μg/mL)和铁离子还原能力(吸光度为0.71),尽管发现与抗坏血酸相比较弱(IC50分别为0.49μg/mL和吸光度为0.93)。
■所有评估的提取物均显示出对白隐球菌和白念珠菌菌株的抗真菌活性(最小抑制浓度值为12.5-25mg/mL),而发现它们对所有测试的细菌菌株的活性可忽略不计。本报告提供了进一步的植物化学研究的初步信息,以分离潜在的抗氧化剂和抗真菌化合物。
UNASSIGNED: Erianthemum aethiopicum Wiens and Polhill (Loranthaceae) is a parasitic plant native to north eastern Africa and Ethiopia. In Ethiopia, it is traditionally used to treat breast swelling, mastitis, morning illnesses and vomiting.
UNASSIGNED: This study aimed to screen the main phytochemical constituents; determine the total amounts of phenolics, flavonoids, and tannins; and evaluate the antimicrobial (against Escherichia coli, Staphylococcus sciuri, Candida glaebosa and Cryptococcus albidus) and antioxidant (against DPPH radical and ferric ion) activities of E. aethiopicum leaves extracts.
UNASSIGNED: Powdered E. aethiopicum leaves were macerated using n-hexane, chloroform, ethyl acetate, ethanol, and methanol. All crude extracts were qualitatively screened for phytochemical identification. The total phenolic, flavonoid, and condensed tannin contents of the chloroform, ethanol, and methanol extracts were determined by UV-Vis spectrophotometry. The n-hexane, chloroform, and methanol extracts were evaluated for their antimicrobial activity against the aforementioned microbes using agar disc diffusion and broth micro-dilution techniques. Chloroform, ethanol, and methanol extracts were also evaluated for antioxidant activity by DPPH and ferric ion reduction antioxidant power (FRAP) assays.
UNASSIGNED: Methanol (17.56 ± 16%) and ethanol (16.45 ± 19%) showed better extraction efficiency. Flavonoids, polyphenols, tannins, terpenoids, saponins, and sterols were detected in all extracts. The highest total content of phenolics (22.63 ± 0.69 mgGAE/gDCE), flavonoids (5.38 ± 0.52 mgCE/gDCE) and tannins (39.18 ± 38 mg CE/g DCE), as milligram of gallic acid and catechin per gram of dried crude extract, were recorded in the methanolic extract. The methanolic extract also presented best anti -DPPH strength (IC50, 4.31 μg/mL) and ferric ion reduction power (absorbance of 0.71) though found weak compared to the ascorbic acid (IC50 of 0.49 μg/mL and absorbance of 0.93, respectively).
UNASSIGNED: All evaluated extracts displayed antifungal activity against both Cryptococcus albidus and Candida glaebosa strains (minimum inhibitory concentration values of 12.5-25 mg/mL), whereas they were found to have negligible activity against all tested bacterial strains. This report provides preliminary information for further phytochemical investigation of Erianthemum aethiopicum to isolate potential antioxidant and antifungal compounds.