今天,比以往任何时候都多,寻找非平凡的生物活性物质来源至关重要。Rumex属的植物值得注意。该属植物从温带气候区的草甸植物群落的优势植物核心中脱颖而出:生长季节短,生物量的密集增加,和不苛刻的生长条件。此外,这种植物属被称为次级酚类化合物的超级生产者。生物活性物质的广泛分布和密集合成使Rumex属植物成为有希望的研究对象。Rumex属的七个物种(R.acetosa,R.acetosella,R.Confertus,R.crispus,R.Maritimus,R.obtusifolius,和血根病)进行了分析。在相对均匀的生长条件下收集植物。为了随后提取和分析酚类化合物,以及抗氧化活性,植物的叶子被使用。R.acetosella,R.crispus,R.Maritimus,R.obtusifolius,和血根虫的特征是酚类化合物的总含量很高(111-131mgg-1)。黄酮类化合物的最大含量是在R.maritimus和R.acetosella的叶子中发现的。同时,根据高效液相色谱与二极管阵列检测(HPLC-DAD)分析,黄酮衍生物(芹菜素和木犀草素)在R.acetosella的叶子中占主导地位,而在其他物种中,确定了黄酮醇的主要衍生物(槲皮素和山奈酚)。R.acetosa的植物,与其他研究物种相比,其特征在于所研究的酚类化合物的含量较低,除了羟基肉桂酸,该物种的含量与类黄酮的含量相当。儿茶素的最大含量是在血根虫中发现的;原花青素-在血根虫中,R.obtusifolius,和R.crispus;和单宁-在R.obtusifolius。薯片提取物具有较高的抗氧化活性,用2,2-二苯基-1-吡啶酰肼(DPPH)测量,2,2'-氮杂-双(3-乙基苯并噻唑啉-6-磺酸(ABTS),和铁还原抗氧化能力(FRAP)测定。此外,对植物酚类成分的评估使得在聚类分析的框架内对植物进行分组成为可能。集群中的分布模式对应于普遍接受的分类法,具有亚属的特征性划分(ACetosa,乙脑,和Rumex)。因此,在制定系统层次结构时,可以将酚类配置文件视为附加的工具方法。
Today, more than ever, the search for non-trivial sources of biologically active substances is critical. Plants of the genus Rumex are noteworthy. Plants of this genus stand out for a number of advantages from the dominant plant core of meadow phytocenoses of the temperate climatic zone: a short growing season, an intensive increase in biomass, and undemanding growth conditions. In addition, this plant genus is known as a super-producer of secondary phenolic compounds. The wide distribution and intensive synthesis of biologically active substances make plants from the genus Rumex a promising object for study. Seven species of the genus Rumex (R. acetosa, R. acetosella, R. confertus, R. crispus, R. maritimus, R.obtusifolius, and R. sanguineus) were analyzed. Plants were collected under relatively uniform growing conditions. For subsequent extraction and analysis of phenolic compounds, as well as antioxidant activity, plants leaves were used. R. acetosella, R. crispus, R. maritimus, R. obtusifolius, and R. sanguineus were characterized by a high total content of phenolic compounds (111-131 mg g-1). The maximum content of flavonoids was found in the leaves of R. maritimus and R. acetosella. At the same time, according to high-performance liquid chromatography with diode-array detection (HPLC-DAD) analysis, derivatives of flavones (apigenin and luteolin) predominated in the leaves of R. acetosella, while in other species, mainly derivatives of flavonols (quercetin and kaempferol) were identified. Plants of R. acetosa, in comparison with other studied species, were characterized by a lower content of the studied groups of phenolic compounds, with the exception of hydroxycinnamic acids, the content of which in this species was comparable to the content of flavonoids. The maximum content of catechins was found in R. sanguineus; proanthocyanidins-in R. sanguineus, R. obtusifolius, and R. crispus; and tannins-in R. obtusifolius. Extracts from R. crispus were characterized by high antioxidant activity, measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2\'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and ferric reducing antioxidant power (FRAP) assays. In addition, the assessment of the phenolic profile of the plant made it possible to group the plants within the framework of cluster analysis. The distribution pattern in the clusters corresponded to the generally accepted taxonomy, with a characteristic division into subgenera (Acetosa, Acetosella, and Rumex). Thus, the phenolic profile can be considered as an additional instrumental approach when drawing up a systematic hierarchy.